摘要
为探讨花鲈(Lateolabrax maculatus)血清和肝脏中代谢酶和抗氧化酶活力以及血清皮质醇含量对盐度骤变的响应,设置两种盐度变化处理方式:1)盐度18骤降至0并持续7 d,然后再骤升至盐度33并持续7 d;2)盐度18骤升至33并持续7 d,然后再骤降至盐度0并持续7 d。分别在盐度骤升和骤降处理后0、0.5、1、3、7 d时取样,测定花鲈血清和肝脏中碱性磷酸酶、酸性磷酸酶、谷丙转氨酶、谷草转氨酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力以及血清皮质醇含量。结果显示,在盐度大幅度骤变条件下,花鲈代谢酶、抗氧化酶活力以及皮质醇含量都发生变化。在大部分骤变条件下,酶活力和皮质醇含量均随时间推移呈现先升高后降低的变化趋势,表明花鲈在盐度骤变时产生了应激反应;但在处理后3~7 d,酶活力和皮质醇含量可恢复至处理前水平,表明花鲈对盐度骤变具有良好的耐受性和适应性。研究结果表明,本实验条件下,花鲈在遭遇养殖环境中盐度骤降变化时,可以较快地恢复,说明该品种对低盐环境有较强的适应能力。
Lateolabrax maculatus is an important economic fish species in China.It is a eurysaline species and can survive and grow in water environment with varieties of salinity.It is of practical significance to study the tolerance and adaptability of L.maculatus,because it usually suffers from sudden increase or decrease of salinity in aquaculture environment.This work investigated the effects of abrupt salinity changes on the activitiy of metabolic enzymes and antioxidant enzymes and cortisol content in liver of L.maculatus by simulating salinity changes of water for its breeding.The salinity was reduced from 18 to 0 and remained for 7 d,and then increased to 33 sharply after acclimation and remained for 7 d.Correspondingly,in the other treatment,the salinity increased from 18 to 33,and then decreased to 0 sharply after acclimation and remained for 7 d.Samples were taken on 0,0.5,1,3,7 d after the start of the experiment,respectively.The activitiy of alkaline phosphatase,acid phosphatase,glutamic-pyruvic transaminase,glutamic-oxalacetic transaminase,superoxide dismutase,catalase,glutathione peroxidase in serum and liver of L.maculatus was tested,and cortisol(COR)content in serum was monitored as well.Results showed that the abrupt salinity changes would have impact on activitiy of metabolic enzymes and antioxidant enzymes,and COR content of L.maculatus.The activitiy of enzymes and COR content showed a trend of increasing firstly and then declining with time prolonging under most situations of abrupt salinity changes.These results showed that L.maculatus had a stress reaction during the abrupt changing of salinity,and could adapt to the change within 3-7 days,which indicated that L.maculatus had a substantial tolerance and adaptability to the abrupt changes in salinity.The changes of morphological characteristics,metabolic enzyme activity,antioxidant enzyme activity and COR content showed that L.maculatus could more rapidly adapt to sudden drop in salinity and was more adaptable to low-salinity environment.
作者
王孝杉
方秀
彭士明
汪睛
施兆鸿
WANG Xiaoshan;FANG Xiu;PENG Shiming;WANG Jing;SHI Zhaohong(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090;Fujian Minwell Industrial CO.,LTD.,Fuding Fujian 355200)
出处
《海洋渔业》
CSCD
北大核心
2021年第3期340-349,共10页
Marine Fisheries
基金
现代农业产业技术体系专项资金(CARS-47)
农业农村部“一带一路”沿线热带国家水产养殖科技合作项目(水产经济动物养殖与模式构建技术输出交流2019)
广西科技基地和人才专项(桂科AD17195024)。
关键词
花鲈
盐度骤变
代谢酶活力
抗氧化酶活力
皮质醇含量
Lateolabrax maculatus
abrupt change in salinity
metabolic enzyme activity
antioxidant enzyme activity
cortisol content
