摘要
以13份国内外菜豆品种为植物材料,测定了其叶片和豆荚中植物凝集素、皂苷、胰蛋白酶抑制剂和植酸4种抗营养因子的含量或活性,并进行了相关性分析及聚类分析。结果表明,菜豆品种抗营养因子分布存在器官特异性,植物凝集素、胰蛋白酶抑制剂、皂苷主要分布在菜豆的豆荚中,植酸在叶片中分布较多。相关性分析得知菜豆的叶片和豆荚的4种抗营养因子的相关性均不显著。聚类分析将菜豆聚为4个品种群,品种群Ⅰ抗营养因子含量较低,可用于低毒菜豆品种选育;品种群Ⅱ抗营养因子含量中等;品种群Ⅲ和Ⅳ抗营养因子含量较高,可用于提取纯化抗营养因子进行进一步研究,或用于抗虫转基因工程研究。
Content or activity of Phaseolus vulgaris agglutinin(PHA),saponin,trypsin inhibitor(TI),phytic acid in leaves and pods of 13 common beans were investigated,and correlation analysis and clustering analysis were carried out. The results showed that the distribution of anti-nutritional factors in common beans existed organ specificity,PHA,TI and saponin mainly distributed in pods,and phytic acid most consisted in leaves. Four kinds of leaves’ and pods’ anti-nutritional factors in common beans had no significant correlation.The test group can be divided into 4 cultivar groups through clustering analysis,variety group Ⅰ had a low content of anti-nutritional factors,which could be used for breeding of low-toxicity common bean varieties;variety group Ⅱ had moderate content of antinutritional factors;the anti-nutritional factors content of varieties group Ⅲ and Ⅳ were higher,which can be used for the further study of anti-nutritional factors’ extraction and purification,or insect-resistant transgenic engineering research.
作者
陈高
朱慧玲
胡格格
刘巧玲
缪宇娴
朱洵
陈禅友
CHEN Gao;ZHU Huiling;HU Gege;LIU Qiaoling;MIAO Yuxian;ZHU Xun;CHEN Chanyou(School of Life Sciences,Hubei Province Engineering Research Center for Legume Plants,Jianghan University,Wuhan 430056,Hubei,China)
出处
《江汉大学学报（自然科学版）》
2021年第4期27-32,共6页
Journal of Jianghan University：Natural Science Edition
基金
湖北省技术创新专项重大项目(2017ABA147)
湖北省食用豆类植物自然科技资源中心开放基金项目(2017-DZY03)。
关键词
菜豆
抗营养因子
凝集素
皂苷
胰蛋白酶抑制剂
植酸
common bean
anti-nutritional factor
lectin
saponin
trypsin inhibitor
phytic acid
