摘要
该文以过硫酸铵(ammonium persulfate,APS)、聚乙烯醇(polyvinyl alcohol,PVA)、3-氨基苯硼酸盐酸盐(3-aminophenylboronic acid hydrochloride,ABA)、苯胺(aniline,AN)为原料,合成聚苯胺基超分子水凝胶(polyaniline based supramolecular hydrogels,PPH)。以戊二醛(glutaraldehyde,GA)为交联剂制备聚苯胺基超分子水凝胶-戊二醛(polyaniline based supramolecular hydrogels-glutaraldehyde,PPH-GA)复合材料作为酶的固定化载体。利用3,5-二硝基水杨酸(dinitrosalicylic acid,DNS)法测定固定化果胶酶活力,计算固定化酶的载酶量,并用单因素变量法考察固定化酶和自由酶在不同酶浓度、温度、pH值、反应时间、果胶浓度下的相对活性,研究固定化果胶酶的酶催化性能。结果表明,固定化酶的最佳催化条件:pH 3.5、反应温度40℃、酶浓度3%、反应时间120 min、果胶浓度2%,此时载酶量为268.1 mg/g。
The supramolecular assembly of polyaniline hydrogel(PPH)was prepared using ammonium persulfate,polyvinyl alcohol,3-aminobenzeneboronic acid hydrochloride,and aniline.Polyaniline based supramolecular hydrogel-glutaraldehyde(PPH-GA)was synthesized using glutaraldehyde as a crosslinking agent.The activity of immobilized pectinase was determined by the dinitrosalicylic acid(DNS)method.The main parameters studied were the following:effects of pH,pectinase concentration,pectin concentration,reaction time,and effect of reaction temperature on the catalytic performance of the immobilized enzyme.The results showed that the immobilized enzyme was 268.1 mg/g at pH 3.5,reaction temperature 40℃,enzyme concentration 3%,reaction time 120 min,pectin concentration 2%.
作者
郝红英
马金玲
詹海鹃
刘万毅
毕淑娴
HAO Hong-ying;MA Jin-ling;ZHAN Hai-juan;LIU Wan-yi;BI Shu-xian(College of Chemistry and Chemical Engineering,State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering,National Demonstration Center for Experimental Chemistry Education,Ningxia University,Yinchuan 750021,Ningxia,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第11期24-28,共5页
Food Research and Development
基金
国家自然科学基金(21467022)
宁夏自然科学基金资助项目(NZ17048)
宁夏“化学工程与技术”国内一流学科建设项目资助(NXYLXK2017A04)
宁夏大学西部一流大学建设项目(ZKZD2017003)。
关键词
水凝胶
果胶
果胶酶
固定化
固定化酶
hydrogel
pectin
pectinase
immobilize
immobilized enzyme
