摘要
甲壳素应用广泛,水产品废弃物往往用作甲壳素的主要制备原料。该研究采用中性蛋白酶去除蛋白质,结合醋酸脱钙的方法处理黄骅特产皮皮虾虾壳,利用单因素和响应面试验对从黄骅特产皮皮虾中制备甲壳素的工艺进行优化。结果表明,在脱蛋白质步骤中,处理时间2 h、温度48℃、酶添加量5000 U为最佳工艺条件。在脱钙步骤中,处理时间1 h、温度20℃、水与醋酸体积比9∶1为最佳工艺条件。经过本研究优化后的甲壳素最终得率为6.87%。
Chitin is widely used,and waste from aquatic products is often the main source of raw material for chitin preparation.In this study,shrimp shells in Huanghua were used as the source material,and chitin preparation technology was optimized using single factor and response surface experiments combining deproteinization with neutral protease,and decalcification using acetic acid.The best conditions for deproteinization were 2 h at 48℃with 5000 U of neutral protease.The best conditions for decalcification were 1 h at 20℃,and the best technological conditions of decalcification was the ratio of water to acetic acid was 9∶1.Using these optimized conditions,chitin yield was 6.87%.
作者
陈依淼
许召旭
司星雨
牛亚男
秦征帆
史兴辰
姜宝杰
CHEN Yi-miao;XU Zhao-xu;SI Xing-yu;NIU Ya-nan;QIN Zheng-fan;SHI Xing-chen;JIANG Bao-jie(College of Science and Engineering,Hebei Agricultural University,Cangzhou 061100,Hebei,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第12期163-170,共8页
Food Research and Development
关键词
甲壳素
虾壳
中性蛋白酶
脱蛋白
醋酸
脱钙
chitin
shrimp shells
neutral protease
deproteinization
acetic acid
decalcification
