摘要
目的检测并分析牙周炎及慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者外周血中分泌白介素-10(interleukin-10,IL-10)的调节性B细胞(B10细胞)占B淋巴细胞的比例(B10细胞比例)及IL-10的水平,探讨B10细胞在牙周炎促发COPD的病理机制中的作用。方法收集2015年1月至2017年6月于首都医科大学附属北京朝阳医院口腔科就诊的牙周炎患者和健康志愿者,呼吸科就诊的COPD患者,将患者分为3组:COPD伴牙周炎组、牙周炎组及健康对照组,3组年龄、性别匹配,每组15例。对3组人群的牙周临床指标和肺功能指标进行统计学分析,同时采集3组人群的外周血,进行细胞刺激和体外培养5和48 h,检测分析外周血中B10细胞比例的差异。扩大样本量,收集2015年1月至2017年6月于首都医科大学附属北京朝阳医院口腔科就诊的牙周炎患者和健康志愿者,以及呼吸科就诊的COPD患者,将患者分为3组:COPD伴牙周炎组、牙周炎组及健康对照组,3组年龄、性别匹配,每组纳入31例,共93例,采用细胞因子微球检测技术测定受试人群血清中IL-10的水平。结果细胞刺激和体外培养5 h,COPD伴牙周炎组B10细胞比例为(0.44±0.11)%,显著低于健康对照组[(0.63±0.14)%]及牙周炎组[(0.62±0.13)%](P<0.01),牙周炎组与健康对照组比较差异无统计学意义(P>0.05)。进行细胞刺激和体外培养48 h后,COPD伴牙周炎组B10细胞比例为(7.59±1.33)%,显著低于牙周炎组[(10.14±2.02)%]和健康对照组[(11.80±1.71)%](P<0.01),同时牙周炎组B10细胞比例显著低于健康对照组(P<0.05)。IL-10的水平在健康对照组、牙周炎组和COPD伴牙周炎组依次降低,COPD伴牙周炎组[(1.95±0.45)ng/L]和牙周炎组[(2.55±0.61)ng/L]均显著低于健康对照组[(3.96±1.15)ng/L](P<0.01),COPD伴牙周炎组与牙周炎组相比差异亦有统计学意义(P<0.01)。结论B10细胞比例降低引起的免疫系统功能紊乱可能参与牙周炎促进COPD的病理进程。
Objective To determine the ratio of ingerleukin(IL)-10 secreting regulatory B cells(B10 cells)and the levels of IL-10 in peripheral blood of periodontitis patients with or without chronic obstructive pulmonary disease(COPD),and to explore the function of B10 cells in the pathogenesis of the interaction between COPD and periodontitis.Methods Forty-five periodontitis patients and healthy people were recruited and divided into three groups:COPD group,COPD+periodontitis group and health controls with 15 people in each group.Peripheral blood samples were collected to determine the B10 population.Another group of 93 periodontitis patients and healthy people were also collected and divided into the same 3 groups with 31 people in each group.Peripheral blood samples were collected to determine the cytokine IL-10.Results After stimulation with lipopolysaccharide(LPS)+phorbol-12-myristate13-acetate+ionomycin+brefeldin A(PIB)for 5 h,the ratio of B10 in COPD+periodontitis group[(0.44±0.11)%]was significantly lower than that in the control group[(0.63±0.14)%]and periodontitis group[(0.62±0.13)%](P<0.01),respectively.The ratio of B10 cells showed no significant difference between periodontitis group and healthy control group(P>0.05).After stimulation with LPS+PIB for 48 h,the ratio of B10 cells in COPD+periodontitis group[(7.59±1.33)%]was significantly lower than that in the periodontitis group[(10.14±2.02)%]and the healthy controls[(11.80±1.71)%](P<0.01),respectively.The ratio of B10 cells in periodontitis group was significantly lower than that in the healthy controls(P<0.05).The levels of IL-10 in periodontitis group[(2.55±0.61)ng/L]and COPD+periodontitis group[(1.95±0.45)ng/L]were significantly lower than that in the control group[(3.96±1.15)ng/L](P<0.01)respectively.Moreover,the level of IL-10 in COPD+periodontitis group was significantly lower than that in the periodontitis group(P<0.01).Conclusions The dysfunction of immune system caused by the decreased proportion of B10 cells might be involved in the pathological process of COPD promoted by periodontitis.
作者
林梅
王悦颖
王蕾
王左敏
Lin Mei;Wang Yueying;Wang Lei;Wang Zuomin(Department of Stomatology,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China)
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2021年第6期525-531,共7页
Chinese Journal of Stomatology
关键词
牙周炎
肺疾病
慢性阻塞性
B10细胞
免疫调节
前瞻性研究
Periodontitis
Pulmonary disease,chronic obstructive
B10 cell
Immunomodulation
Prospective studies
