摘要
研究旨在确定云南省蓝莓主产区发生的蓝莓根癌病病原菌种类,并进行田间防治药剂筛选。采集云南省蓝莓主产区蓝莓根癌病样品,通过发病症状、菌落形态观察、致病性测定、Biolog分析、16S rDNA序列分析比较进行系统鉴定。在发病严重的蓝莓基地采用噻霉酮、申嗪霉素等8种药剂进行田间防治试验。从蓝莓根癌病样品中分离、纯化获得的菌株L-11接种向日葵、番茄、蓝莓植株均能发病,且发病症状与田间症状相同,重新分离得到形态相同的菌株。16S rDNA序列分析和系统进化树结果表明,L-11菌株序列与根癌土壤杆菌(Agrobacterium tumefaciens)菌株Ell-7(登录号FJ613554.1)同源性高达97.65%,与根癌土壤杆菌处于同一分支上。菌株L-11利用ipt基因的特异性引物AtP3F/AtP3R能扩增出155 bp的特异性片段,进一步证实蓝莓根癌病由根癌土壤杆菌引起。田间药效试验结果表明,1.5%噻霉酮水乳剂对蓝莓根癌病有较好的防治效果,相对防效为52%。这是云南首次报道由根癌土壤杆菌引起蓝莓根癌病。
The study aims to determine the species of blueberry crown gall pathogenic bacteria currently occurring in the main blueberry producing areas of Yunnan Province and screen the field control agents.Collecting blueberry crown gall disease samples from the main blueberry production areas in Yunnan, the symptoms of the disease, colony morphology, pathogenicity test, Biolog analysis and 16 S rDNA sequence analysis were carried out for systematic identification. The agent screening test was conducted at the blueberry base with serious disease, and eight kinds of pesticides such as thiazinone and shenzemycin were used for field control experiments. The strain L-11 isolated and purified from blueberry crown gall disease samples could infect sunflower, tomato and blueberry plants, and the symptoms were similar to those in field. The strain with identical morphology was obtained by re-isolation. 16 S r DNA sequence analysis and phylogenetic tree analysis showed that the sequence of L-11 strain and Agrobacterium tumefaciens strain Ell-7(accession number:FJ613554.1) homology was up to 97.65%, and L-11 was on the same branch as A. tumefaciens. The specific fragment of 155 bp was amplified by the ipt specific primer AtP3 F/AtP3 R in L-11, which further confirmed that blueberry crown gall disease was caused by A. tumefaciens. The field efficacy test results showed that 1.5%thiazinone emulsion in water had a better control effect on blueberry crown gall disease, with a relative control effect of 52%. This is the first report of blueberry crown gall disease caused by A. tumefaciens in Yunnan.
作者
张荣琴
万效琰
张晋豪
代真林
郑建立
崔兴国
赵项武
魏兰芳
姬广海
Zhang Rongqin;Wan Xiaoyan;Zhang Jinhao;Dai Zhenlin;Zheng Jianli;Cui Xingguo;Zhao Xiangwu;Wei Lanfang;Ji Guanghai(Key Laboratory of Agriculture Biodiversity for Plant Disease Management of the Ministry of Education/Yunnan Agricultural University,Kunming 650201;Honghe Tropical Agricultural Science Institute of Yunnan,Hekou Yunnan 661300;Qujing Jiawo Modern Agriculture Co.,Ltd.,Qujing Yunnan 655000)
出处
《中国农学通报》
2021年第16期119-126,共8页
Chinese Agricultural Science Bulletin
基金
国家重点研发计划项目“主要经济作物重要及新成灾害绿色综合防控技术”(2019YFD1002000)
云南省“万人计划”产业技术领军人才专项(YNWR-CYJS-2019-046)
云南省重点研发(农业领域)计划项目“蓝莓病虫害诊断和绿色防控综合体系建设”(2018BB016)。
关键词
蓝莓根癌病
病原菌
分离
鉴定
根癌土壤杆菌
药剂筛选
blueberry crown gall disease
pathogenic bacteria
isolation
identification
Agrobacterium tumefaciens
pharmacy screening
