摘要
从曼地亚红豆杉树皮中分离得到1株链格孢属(Alternaria sp.)内生真菌MF5。初期马铃薯葡萄糖(PD)培养基液体培养结果显示,MF5的发酵产物中检测到的紫杉醇质量浓度约为0.58 mg/L。为了提高MF5发酵产物中紫杉醇含量,考察添加CuSO_(4)、茉莉酸甲酯(MeJA)、赤霉素(GA_(3))和红豆杉浸出液对MF5产紫杉醇的影响。结果表明:添加4%红豆杉浸出液,对MF5进行发酵培养,发酵产物中紫杉醇质量浓度为(2.47±0.07)mg/L;在此基础上,添加CuSO_(4)、MeJA、GA_(3)3种诱导子也能够提高MF5产紫杉醇的能力,且同时添加3种诱导子时,MF5发酵物中紫杉醇含量最高,达到(12.58±0.80)mg/L。本研究证实了3种诱导子和红豆杉浸出液均可促进内生真菌代谢产生紫杉醇,为后续真菌发酵法生产紫杉醇打下基础。
The endophytic fungus MF5,which belongs to Alternaria sp.,was isolated from the bark of Taxus media.Preliminary potato glucose medium(PD)cultural results showed that paclitaxel mass concentration was about 0.58 mg/L,nurtured from MF5 fermentation.To improve the content of paclitaxel in MF5 fermentation products,the effects of different elicitors(yew crude extracts,CuSO_(4),MeJA and GA_(3))on the production of paclitaxel were explored.Results showed that the content of paclitaxel in the fermentation products was(2.47±0.07)mg/L by adding 4%yew crude extracts.In addition,the increase of CuSO_(4),MeJA and GA_(3) elicitors could also increase the production of paclitaxel by MF5.Based on the above,when the three elicitors and yew crude extracts were added together,the production of MF5 strains was significantly increased,which was reached(12.58±0.80)mg/L.This research confirmed that these three elicitors and yew crude extracts could promote the metabolism of endophytic fungus to produce paclitaxel,laying the academic foundation of subsequent studies of paclitaxel productions by fungal fermentation.
作者
陈国晶
杨宁
吴文静
喻静
金磊磊
张爱华
陈集双
CHEN Guojing;YANG Ning;WU Wenjing;YU Jing;JIN Leilei;ZHANG Aihua;CHEN Jishuang(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;College of Oceanography,Hohai University,Nanjing 210098,China)
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2021年第4期535-539,共5页
Journal of Nanjing Tech University(Natural Science Edition)
基金
江苏先进生物制造协同创新中心(XTD1825)
国家自然科学基金(31560079)。
