摘要
超分辨荧光显微技术一直是研究热点,各种超分辨荧光成像技术的出现打破了光学行射极限,将空间分辨率提高到纳米尺度。但随着时间、空间分辨率要求的不断提高,目前超分辨荧光显微技术仍然面临着空间分辨率、时间分辨率和视场存在三角制约关系及探测灵敏度较低的问题。随着近年来基于量子关联的新型成像机制的发展,基于荧光量子特性与量子关联成像的超分辨荧光显微镜应运而生,新成像模式及新物理量的引入不仅增加了信息量也提高了图像信息获取的效率,为超分辨荧光显微技术的研究提供了新思路。介绍了目前超分辨荧光显微镜的技术原理、优缺点与面临的主要技术问题,以及基于量子关联的新型超分辨荧光显微镜,并探讨了未来超分辨荧光显微镜的发展方向,以期为该领域的科学研究提供参考。
Super-resolution fluorescence microscopy has always been a hot research field.The emergence of various super-resolution fluorescence imaging techniques has broken the optical diffraction limit and improved the spatial resolution to nanoscale.However,for higher requirements on temporal and spatial resolution,the current super-resolution fluorescence microscopes still face two problems of the triangular constraint relation of spatial resolution,temporal resolution and field of view,and low detection sensitivity.However,with the recent development of the new imaging mechanism based on quantum correlation,the super-resolution fluorescence microscopes based on fluorescence quantum property and quantum correlation imaging have been developed.The introduction of new imaging modes and new physical quantities not only increases the amount of information but also improves the efficiency of image information acquisition.A new research route for the development of super resolution fluorescence microscopy is provide.This review introduces the technological principle,advantages and disadvantages of the current super-resolution fluorescence microscopes as well as the main technical problems faced by them.Some new super resolution fluorescence microscopes based on quantum correlation are introduced and the future development direction of super resolution fluorescence microscopy is discussed.It is expected that this review can provide some references for the researches in this field.
作者
李文文
王中阳
Li Wenwen;Wang Zhongyang(Center for Fundamental Interdisciplinary Research,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;School of Microelctronics,University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2021年第10期158-172,共15页
Laser & Optoelectronics Progress
关键词
成像系统
荧光显微镜
超分辨显微镜
量子关联
探测灵敏度
时间分辨率
imaging systems
fluorescence microscope
super-resolution microscope
quantum correlation
detection sensitivity
temporal resolution
