摘要
目的:分析山东新泰地区人乳头瘤病毒(HPV)感染状况及基因亚型分布特征,为该地区提供预防、诊治宫颈癌的理论依据。方法:采用聚合酶链反应PCR-反向杂交技术对6410例宫颈脱落细胞标本进行HPV基因分型检测。结果:6410例样本中检出HPV阳性样本1406例,阳性率21.93%,共检出HPV2120株。其中检出HPV高危型1452株(68.49%),低危型668株(31.51%);在被检测的17种高危型HPV亚型中常见的是16、52、58型,在低危型HPV亚型中常见的是6、11、81型,各亚型在不同的年龄组中排列分布各不相同;HPV感染中单一感染率为42.55%,多重感染率为57.45%,其中以二重感染为主;在不同的年龄组中HPV检出率最高的为16~20岁组(35.48%),其次是20~30岁组(32.77%),单一感染率最高的是51~60岁组(占本组72.87%),多重感染率最高的是16~20岁组(占本组63.64%)。各年龄组HPV感染率差异有统计学意义(P<0.001)。结论:新泰地区HPV感染高危型以16、52、58为主,以单一型感染和二重感染为主,各年龄组HPV亚型分布特征各不相同,30岁以下人群应作为HPV监测重点人群,同时应根据本地区HPV分布型别及年龄差异,针对性的选择适合本地区的HPV疫苗,对HPV感染早期预防和治疗具有重要的临床价值。
Objective:To analyze the human papillomavirus(HPV)infection status and the distribution characteristics of genotypes in Xintai,Shandong,and provide theoretical basis for the prevention,diagnosis and treatment of cervical cancer in this area.Methods:Using polymerase chain reaction PCR-reverse hybridization technology to perform HPV genotyping on 6410 cervical exfoliated cell specimens.Results:Among 6410 samples,1406 HPV-positive samples were detected,with a positive rate of 21.93%.A total of 2120 HPV strains were detected,of which 1452 high-risk HPV strains(68.49%)and 668 low-risk HPV strains(31.51%)were detected.Among the 17 high-risk HPV subtypes,types 16,52,and 58 are common,and among the low-risk HPV subtypes,types 6,11,and 81 are common.Each subtype is arranged differently in different age groups.The single infection rate in HPV infection is 42.55%,and the multiple infection rate is 57.45%,among which the double infection is the main one.Among different age groups,the HPV detection rate is highest in the 16-20 year-old group(35.48%),followed by It is the 20-30 year-old group(32.77%),the 51-60 year old group has the highest single infection rate(accounting for 72.87%of this group),and the 16-20 year old group has the highest multiple infection rate(accounting for 63.64%of this group).There is a statistically significant difference in HPV infection rates among age groups(P<0.001).Conclusion:The high-risk subtypes of HPV infection in Xintai area are mainly 16,52,and 58.The main types are single infection and double infection.The distribution characteristics of HPV subtypes in each age group are different.People under 30 years old should be the key population for HPV monitoring.At the same time,according to the HPV distribution types and age differences in the region,the targeted selection of HPV vaccines suitable for the region has important clinical value for the early prevention and treatment of HPV infection.
作者
孔祥菊
KONG Xiang-ju(Department of Clinical Laboratory,Xintai People's Hospital,Shandong Xintai 271200)
出处
《医学检验与临床》
2021年第6期22-26,共5页
Medical Laboratory Science and Clinics
关键词
人乳头瘤病毒
基因分型
感染
分布特点
Human papillomavirus
Genotyping
Infection
Distribution characteristics
