摘要
利用油菜50K SNP芯片对39份甘蓝型油菜临保系进行遗传多样性分析,绘制SNP标记染色体密度图.结果表明:SNP标记并非均匀分布在每条染色体上,甚至有些区段的SNP标记空缺;通过MEGA5和SNPhylo分别绘制出聚类分析图和遗传进化树,其分类结果趋于一致,但遗传进化树展示聚类结果更为直观.使用MEGA5聚类分析,设置阈值为0.3,可将临保系材料分为4个类群,聚类结果和花色及品质的考察结果基本一致.
The genetic diversity of 39 temporary maintainer lines of rapeseed(Brassica napus L.)was analyzed by using 50K SNP microarray,and a chromosome density map of SNP markers was plotted.The results showed that SNP markers were not evenly distributed on every chromosome,and some SNP markers were absent in some regions of the chromosome.A cluster analysis diagram and a genetic evolutionary tree were drawn with MEGA5 and Snphylo,respectively,and their classification results tended to be consistent with each other,but the genetic evolutionary tree gave a more visual representation of the clustering results.MEGA5 analysis,with the threshold set at 0.3,clustered the temporary maintainers into 4 groups,and the results were basically consistent with the inspection results of color and quality.
作者
陈雪
王瑞
张胜森
郭青青
李加纳
CHEN Xue;WANG Rui;ZHANG Sheng-sen;GUO Qing-qing;LI Jia-na(School of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第8期36-41,共6页
Journal of Southwest University(Natural Science Edition)
基金
科技部国家重点研发计划项目(2016YFD0101300).
关键词
甘蓝型油菜
SNP芯片
临保系
Brassica napus L.
SNP chip
temporary maintainer
