摘要
目的:采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(UPLC-Q-Orbitrap HRMS)和网络药理学方法探索桑姜感冒注射液治疗普通感冒的物质基础和作用机制。方法:采用UPLC-Q-Orbitrap HRMS鉴定桑姜感冒注射液中的化学成分,流动相乙腈(A)-0.1%甲酸水溶液(B)梯度洗脱(0~10 min,4%~15%A;10~35 min,15%~30%A;35~45 min,30%~33%A;45~55 min,33%~60%A;55~58 min,60%A),流速0.2 m L·min^(-1),电喷雾离子源(ESI),正、负离子模式下扫描,扫描范围m/z 100~1500。利用中药系统药理学数据库和分析平台(TCMSP)和Gene Cards 5.0数据库筛选并预测桑姜感冒注射液化学成分的潜在作用靶点;利用STRING 11.0数据库和Cytoscape 3.7.2软件构建蛋白质-蛋白质相互作用(PPI)网络模型;使用Metascape3.5,Reactome数据库和京都基因与基因组百科全书(KEGG)对潜在靶点进行基因本体(GO)分析和通路分析;利用Cytoscape3.7.2软件建立"药材-成分-核心靶点"网络。结果:分析鉴定了桑姜感冒注射液中54种成分,并以此为基础筛选出80个桑姜感冒注射液治疗普通感冒的潜在靶点。桑姜感冒注射液通过作用于白细胞介素(IL)-6,肿瘤坏死因子(TNF)和IL-10等靶点,以及IL-17信号通路,TNF信号通路,细胞因子受体相互作用等信号通路发挥治疗作用。结论:桑姜感冒注射液可能通过作用于炎症和免疫系统等相关的靶点及通路,干预炎性细胞因子的表达,整体调节机体的免疫功能,从而发挥治疗作用。
Objective:To explore the material basis and mechanism of Sangjiang Ganmao injection(SG)in the treatment of common cold by ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS)and network pharmacology.Method:UPLC-Q-Orbitrap HRMS was used to identify the chemical components of SG with mobile phase of acetonitrile(A)-0.1%formic acid aqueous solution(B)for gradient elution(0-10 min,4%-15%A;10-35 min,15%-30%A;35-45 min,30%-33%A;45-55 min,33%-60%A;55-58 min,60%A),flow rate of 0.2 mL·min^(-1),electrospray ionization(ESI)and scanning range of m/z 100-1500 under positive and negative ion modes.Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards5.0 database were used to screen and predict the potential targets of chemical components in SG,STRING 11.0 database and Cytoscape 3.7.2 software were used to construct protein-protein interaction(PPI)network model,gene ontology(GO)analysis and pathway analysis were performed on potential targets by Metascape 3.5,Reactome database and Kyoto Encyclopedia of Genes and Genomes(KEGG),Cytoscape 3.7.2 software was used to build the network of"herbs-ingredients-key targets".Result:A total of 54 components in SG were identified,and 80 potential targets of SG for treatment of common cold were predicted and screened based on this.SG exerted therapeutic effects by acting on targets such as interleukin(IL)-6,tumor necrosis factor(TNF)and IL-10,and signaling pathways such as IL-17 signaling pathway,TNF signaling pathway and interaction of cytokine receptors.Conclusion:SG may interfere with the expression of inflammatory cytokines by acting on related targets and pathways such as inflammation and immune system,and regulate the immune function of the body as a whole,thereby exerting a therapeutic effect.
作者
彭诚艺
曾逸佳
熊海均
耿福能
黄勤挽
张大永
PENG Cheng-yi;ZENG Yi-jia;XIONG Hai-jun;GENG Fu-neng;HUANG Qin-wan;ZHANG Da-yong(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Gooddoctor Pharmaceutical Group Co.Ltd.,Chengdu 610071,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第14期155-163,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
成都中医药大学“杏林学者”学科人才科研提升计划项目(QNXZ2018023,XSGG2019008)。
关键词
桑姜感冒注射液
桑菊饮
普通感冒
网络药理学
物质基础
炎症
免疫系统
Sangjiang Ganmao injection
Sangjuyin
common cold
network pharmacology
material basis
inflammation
immune system
