摘要
为了探讨高温胁迫对苜蓿愈伤组织内源激素含量及比值的影响,试验将经600 Gy和800 Gy剂量^(60)Co-γ射线辐射的龙牧806紫花苜蓿种子种植后收获的第二代苜蓿种子进行幼苗培养,筛选变异植株,对变异植株的叶片进行愈伤组织诱导,获得G3(800 Gy)和G4(600 Gy)苜蓿愈伤组织,以未经^(60)Co-γ射线辐射的龙牧806苜蓿种子获得的植株叶片愈伤组织为对照(CK),对3种苜蓿愈伤组织进行45℃高温胁迫,利用高效液相色谱法对苜蓿愈伤组织中吲哚乙酸(IAA)、赤霉素(GA3)和脱落酸(ABA)含量进行测定,计算ABA/IAA、ABA/GA3和耐热系数(HTC),分析高温胁迫1,2,3,4,5 h苜蓿愈伤组织的内源激素含量及比值变化,最后利用隶属函数法对3种苜蓿愈伤组织抗高温能力进行综合评价。结果表明:随着高温胁迫时间的延长,CK、G3和G4苜蓿愈伤组织中IAA和GA3含量呈降低趋势,ABA含量、ABA/IAA和ABA/GA3呈增加趋势;且除高温胁迫5 h的G4愈伤组织IAA含量外,G3和G4苜蓿愈伤组织IAA、GA3、ABA含量均高于CK苜蓿愈伤组织;G3苜蓿愈伤组织隶属函数值最高,其抗高温能力最强。说明在应对高温逆境时ABA、IAA和GA3可参与调节高温胁迫反应,且辐射可以提高苜蓿愈伤组织抗高温能力。
In order to study the effect of high temperature stress on the content and ratio of endogenous hormones in alfalfa callus, the second generation alfalfa seeds of Longmu 806 irradiated by ^(60)Co-γ radiation of 600 Gy和800 Gy were used for seedling culture. The mutant plants were selected and the leaves of the mutant plants were induced to obtain G3(800 Gy) and G4(600 Gy) alfalfa callus. The leaves of Longmu 806 alfalfa seeds without ^(60)Co-γ radiation were used for callus induction, which was the control(CK) alfalfa callus. The three kind of alfalfa callus were subjected to 45℃ high temperature stress. Then the contents of indoleacetic acid(IAA), gibberellin(GA3), and abscisic acid(ABA) in alfalfa callus were determined by HPLC. The ratio of ABA/IAA, ABA/GA3, and heat tolerance coefficient(HTC) were calculated. The changes of endogenous hormone content and ratio in alfalfa callus under high temperature stress for 1, 2, 3, 4, 5 h were analyzed. Finally, the high temperature resistance of three alfalfa calli was evaluated by membership function method. The results showed that with the increase of high temperature stress time, the contents of IAA and GA3 in CK, G3 and G4 alfalfa callus decreased, but the contents of ABA and the ratio of ABA/IAA and ABA/GA3 increased. The contents of IAA, GA3, ABA in G3 and G4 calli were higher than those in control callus, except for IAA content in G4 calli under high temperature stress for 5 h.The membership function value of G3 alfalfa callus was the highest, and it had the strongest resistance to high temperature. It indicated that ABA, IAA and GA3 could participate in the regulation of high temperature stress response, and radiation could improve the high temperature resistance of alfalfa callus.
作者
朱琨
李波
邬婷婷
ZHU Kun;LI Bo;WU Tingling(College of Agriculture,Forestry and Life Sciences/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第12期102-106,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省省属高等学校基本科研业务费科研项目(135409216)。
