摘要
目的探讨miR-1275对宫颈癌Hela细胞生长影响及其机制。方法将体外培养的Hela细胞分为miR-NC组、miR-1275组、anti-miR-NC组和anti-miR-1275组,采用RT-PCR检测miR-1275的表达,CCK-8法评估Hela细胞增殖活力,Transwell小室法测定Hela细胞迁移和侵袭能力,流式细胞仪检测细胞凋亡率。生物信息学软件预测miR-1275靶基因,双荧光素酶报告基因实验验证miR-1275和FHIT的靶向关系,Western blot检测FHIT蛋白的表达。结果上调miR-1275表达显著增加Hela细胞活力、侵袭和迁移能力(P<0.05),降低细胞凋亡率(P<0.05),而下调miR-1275表达具有相反作用。miR-1275与FHIT 3’UTR存在互补的结合位点,FHIT是miR-1275的靶基因。上调miR-1275表达显著抑制FHIT蛋白表达(P<0.05),而下调miR-1275表达显著促进FHIT蛋白表达(P<0.05)。结论miR-1275可促进宫颈癌Hela细胞增殖、侵袭和迁移,并抑制细胞凋亡,其分子机制可能与靶向调控FHIT表达有关。
Objective To investigate the effect of miR-1275 on the growth of cervical cancer Hela cells and its mechanism.Methods Hela cells cultured in vitro were divided into miR-NC group,miR-1275 group,anti-miR-NC group and anti-miR-1275 Group.The expression of miR-1275 was detected by RT-PCR,Cck-8 assay was used to evaluate the proliferation activity of Hela cells was evaluated by CCK-8 assay,the migration and invasion ability of Hela cells was measured by Transwell assay,and cell apoptosis rate was detected by flow cytometry.Bioinformatics software predicted miR-1275 target genes,and dual luciferase reporter assays validated the targeting relationship between miR-1275 and FHIT,and the expression of FHIT protein was detected by Western blot.Results of miR-1275 up-regulation significantly increased Hela cell viability,invasion and migration abulity(P<0.05),and decreased apoptosis rate(P<0.05),while miR-1275 down-regulation had an opposite effect.BFHIT 3'UTR has a binding site that was complementary to miR-1275,and FHIT was the target gene of miR-1275.miR-1275 up-regulation significantly inhibited FHIT protein expression(P<0.05),while miR-1275 down-regulation significantly promoted FHIT protein expression(P<0.05).Conclusion miR-1275 can promote cervical cancer Hela cells proliferation,invasion and migration,and inhibit cell apoptosis.Its molecular mechanism may be related to the targeted regulation of FHIT expression.
作者
范晓丽
王义娜
张青青
FAN Xiaoli;WANG Yina;ZHANG Qingqing(Pingmei Shenma Medical Group General Hospital,Pingdingshan,467000)
出处
《实用癌症杂志》
2021年第7期1054-1057,共4页
The Practical Journal of Cancer
