摘要
为研究饲料维生素C(Vc)对急性降温后花鲈(Lateolabrax maculatus)免疫、抗氧化及抗应激的影响,以含有不同水平Vc(6.85、46.90、88.24、167.43、329.21和658.69 mg/kg)的饲料饲养花鲈56 d后,进行急性降温(从33℃水体转入27℃水体)处理24 h和48 h。结果显示,降温24 h和48 h时,各组血清补体3(C3)水平高于0h,而补体4(C4)水平低于0h。饲料Vc在6.85~167.43mg/kg时,血清免疫球蛋白M(IgM)水平随降温时间的增加,在24 h和48 h显著高于0 h(P<0.05);当饲料Vc含量高于167.43 mg/kg时,各时间点Ig M差异不显著。血清溶菌酶(LZM)活性在降温48 h后显著低于降温前(P<0.05),而各Vc添加组显著高于对照组(P<0.05)。肝脏丙二醛(MDA)含量在降温后显著高于降温前,但在降温后随着饲料Vc水平的升高而显著降低(P<0.05)。肝脏总超氧化物歧化酶(T-SOD)含量随降温时间的增加,先增加后降低。在各时间点,花鲈肝脏谷胱甘肽(GSH)含量都随着饲料Vc水平的升高而升高。当饲料Vc为6.85mg/kg时,肝脏血红素氧合酶(HO-1)活性在降温后显著高于降温前(P<0.05);当饲料Vc为88.24~167.43 mg/kg时,肝脏HO-1活性在降温后显著低于降温前(P<0.05)。花鲈血清热休克蛋白(HSP70)水平在降温后显著高于降温前(P<0.05),而当降温时间达到48h时,各Vc添加组HSP70含量显著低于对照组。研究表明,饲料中适量的Vc(46.90~167.43 mg/kg)能提高花鲈肝脏的抗氧化能力,减轻脂质过氧化水平,缓解花鲈急性降温引起的应激反应。
The purpose of this study was to investigate the effects of dietary vitamin C on the immunity,antioxidant capacity,and stress tolerance of juvenile Lateolabrax maculatus under acute cooling stress.An 8-week feeding trial was conducted with experimental diets containing 6.85,46.90,88.24,167.43,329.21,and 658.69 mg/kg vitamin C(Vc).Some fish were then exposed to acute cooling stress for 24 h and 48 h(transferred from 33℃water to 27℃water).The results showed that the serum levels of Complement 3(C3)in each group after cooling were higher than before cooling,while the levels of serum Complement 4(C4)showed the opposite pattern.When dietary Vc was 6.85~167.43 mg/kg,the levels of serum immunoglobulin M(IgM)at 24 h and 48 h were higher than at 0 h(P<0.05).However,there were no differences among the treatments with higher dietary Vc supplementation(329.21~658.69 mg/kg;P>0.05).After cute cooling for 48 h,the levels of serum lysozyme(LZM)were lower than at 0 h,but the Vc-supplemented groups were higher than the control group(P<0.05).The concentration of hepatic malondialdehyde(MDA)were higher in all groups after cooling stress than before cooling stress,but decreased with increasing levels of dietary Vc(P<0.05).Total superoxide dismutase(T-SOD)activity initially increased but then decreased with greater cooling time,and the glutathione(GSH)content increased with increasing dietary Vc supplementation.Heme oxygenase-1(HO-1)activity in the 6.85 mg/kg Vc group was higher after cooling stress,but the groups supplemented with 88.24 to 167.43 mg/kg Vc had lower activities after cooling(P<0.05).The levels of heat shock proteins(HSP70)were higher before acute cooling,and when cooled for 48 h,HSP70 levels in the Vc-supplemented groups were lower than the control group(P<0.05).These results suggest that dietary Vc supplementation(46.90~167.43 mg/kg)may improve antioxidant capacity,reduce lipid peroxidation,and enhance the resistance to acute cooling in L.maculatus.
作者
孙敬诚
李潇
鲁康乐
王玲
宋凯
张春晓
SUN Jingcheng;LI Xiao;LU Kangle;WANG Ling;SONG Kai;ZHANG Chunxiao(Xiamen Key Laboratory of Feed Testing and Safety Evaluation,Fisheries College,Jimei University,Xiamen,Fujian 361021,China)
出处
《渔业科学进展》
CSCD
北大核心
2021年第4期126-133,共8页
Progress in Fishery Sciences
基金
财政部和农业农村部:国家现代农业产业技术体系(CARS-47)资助。
关键词
花鲈
维生素C
急性降温
抗应激
Lateolabrax maculatus
Vitamin C
Acute cooling stress
Stress tolerance
