摘要
目的探究阿尔茨海默症(AD)患者肠道微生态结构与认知功能的相关性。方法选取2016年10月~2019年10月在遵化市人民医院接受治疗的88例AD患者为研究组,同期在本院进行体检的80例健康者为对照组,采用简易精神状态评价量表(MMSE)评价研究对象认知功能,采用高通量测序检测两组肠道微生物状况,计算Shannon指数和Chaol指数,并记录各菌群的相对丰度。结果研究组MMSE得分低于对照组,差异有统计学意义(P<0.05);研究组放线菌门、拟杆菌门、放线菌科、乳杆菌科、拟杆菌科、双歧杆菌属、乳杆菌属、拟杆菌属相对丰度及Shannon指数、Chaol指数均低于对照组,厚壁菌门、变形菌门、子囊菌门、假单胞菌科、葡萄球菌科、肠杆菌科、梭菌科、隐球酵母科、梭状芽胞杆菌属、假单胞菌属、葡萄球菌属、变形杆菌属、假丝酵母菌属相对丰度均高于对照组,差异均有统计学意义(P<0.05);研究组拟杆菌门、放线菌门、拟杆菌科、乳杆菌科、放线菌科、拟杆菌属、乳杆菌属、双歧杆菌属相对丰度及Chaol指数、Shannon指数与MMSE得分呈正相关(P<0.05),子囊菌门、变形菌门、厚壁菌门、隐球酵母科、肠杆菌科、葡萄球菌科、假单胞菌科、梭菌科、假丝酵母菌属、变形杆菌属、葡萄球菌属、假单胞菌属、梭状芽孢杆菌属相对丰度与MMSE得分呈负相关(P<0.05)。结论AD患者认知功能与肠道微生态结构相关,患者肠道微生态结构异常会出现严重的认知障碍。
Objective To explore the correlation between the intestinal microecological structure and cognitive function in patients with Alzheimer’s disease( AD). Methods 88 patients with AD in Zunhua People’s Hospital from October 2016 to October2019 were assigned into the research group. 80 persons undergoing healthy physical examinations were assigned into the control group.Mini-mental state examination( MMSE) was used to evaluate the cognitive function. High-throughput sequencing was used to detect the intestinal microbes. Shannon index and Chaol index were calculated,and the relative abundance of each flora were recorded.Results The score of MMSE in the research group was lower than that in the control group( P < 0. 05). In the research group,the relative abundances,Shannon index and Chaol index of Actinobacteria,Bacteroides,Actinomycetes,Lactobacillus,Bacteroides,Bifidobacterium,Lactobacillus,Bacteroides were lower than those in the control group( P < 0. 05),the relative abundances of Firmicutes,Proteobacteria,Ascomycota,Pseudomonas,Staphylococcus,Enterobacteriaceae,Clostridia,Cryptococcus,Clostridium,Pseudomonas,Staphylococcus Genus,Proteus,Candida were higher than those in the control group( P < 0. 05). In the research group,the relative abundance,Chaol index,Shannon index of Bacteroides,Actinobacteria,Bacteroides,Lactobacillus,Actinomycetes,Bacteroides,Lactobacillus,Bifidobacterium were positively correlated with MMSE score( P < 0. 05). The relative abundance of Ascomycota, Proteobacteria, Firmicutes, Cryptococcus, Enterobacteriaceae, Staphylococcus, Pseudomonas, Clostridia,Candida,Proteus,Staphylococcus,Pseudomonas,Clostridium were negatively correlated with MMSE score( P < 0. 05). Conclusion The cognitive function of AD patients is related to the intestinal microecological structure,and patients with abnormal intestinal microecological structure will have severe cognitive impairment.
作者
白进川
宋凯杰
刘丽
张宏杰
高峰
Bai Jinchuan;Song Kaijie;Liu Li;Zhang Hongjie;Gao Feng(Department of Blood Transfusion,Zunhua People's Hospital,Zunhua 064200;Department of Laboratory,Beijing Henghe Integrated Chinese and Western Medicine Hospital,Beijing 100005;Department of Laboratory,Kailuan General Hospital,Tangshan 063000;Department of Laboratory Diagnosis,Zunhua People's Hospital,Zunhua 064200)
出处
《国际老年医学杂志》
2021年第4期199-202,共4页
International Journal of Geriatrics
基金
河北省医学科学研究课题项目(20191740)。
关键词
阿尔茨海默症
肠道微生态结构
认知功能
相关性
Alzheimer's disease
Intestinal microecological structure
Cognitive function
Correlation
