摘要
采用免疫组织化学(IHC)、蛋白免疫印迹(Western blot)和实时荧光定量(qRT-PCR)检测方法对初生、幼年、成年及老年牦牛肺中CTGF和FGF-2的准确分布位置及其表达量进行研究。IHC结果显示,CTGF主要分布于终末细支气管黏膜上皮克拉拉细胞和管壁平滑肌细胞,肺动脉内皮细胞以及中膜平滑肌细胞;且在初生组和老年组表达量最高;FGF-2主要分布于终末细支气管管壁平滑肌和肺动脉管壁平滑肌,在所有年龄段都维持较高的表达水平。Western blot结果显示,CTGF和FGF-2在不同年龄组牦牛肺均能检测到表达,CTGF在初生组和老年组表达量显著高于幼年组和成年组(P<0.05);FGF-2在初生组和幼年组表达量显著高于成年组和老年组(P<0.05)。qRT-PCR结果显示,CTGF在初生组转录水平最高,且各年龄组之间比较差异均显著(P<0.05);FGF-2在老年组转录水平最高,其次为成年组,与其他组相比差异均显著(P<0.05)。CTGF和FGF-2对牦牛肺适应性结构的形成和适应性过程有关,在初生和老年阶段作用较为显著。
The exact distribution and expression of CTGF and FGF-2 in the lungs of newborn,young,adult and old yaks were studied by immunohistochemistry(IHC),Western blot and real-time fluorescence quantitative(qRT-PCR).The results of IHC showed that CTGF was mainly distributed in terminal bronchiole epithelium Clara cells and smooth muscle cells,pulmonary artery endothelial cells and medium membrane smooth muscle cells;The expression levels were the highest in the newborn and elderly groups;FGF-2 was mainly distributed in the smooth muscle of the terminal bronchiole and the smooth muscle of the pulmonary artery,and maintained a high expression level in all age groups.The results of WB showed that the expression of CTGF and FGF-2 could be detected in the lungs of yaks in different age groups,the expression of CTGF in newborn group and elderly group was significantly higher than that in young group and adult group(P<0.05),and the expression of FGF-2 in the newborn group and young group was significantly higher than that in adult group and old group(P<0.05).The results of qRT-PCR showed that the transcriptional level of CTGF was the highest in the newborn group,and there were significant differences among different age groups,while the transcriptional level of FGF-2 in the elderly group was the highest,followed by the adult group,and there were significant differences compared with other groups(P<0.05).CTGF and FGF-2 are related to the formation of adaptive structure and adaptive process of yak lung,and play a significant role in newborn and old age.
作者
李月娇
崔燕
张倩
何俊峰
LI Yuejiao;CUI Yan;ZHANG Qian;HE Junfeng(College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730000, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2021年第7期2025-2033,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31960687)
甘肃农业大学青年导师基金资助项目(GAU-QNDS-201708)。
