摘要
目的探究微小RNA361-5p(miR-361-5p)在肺结核病人血清中水平及意义。方法选取2017年3月至2019年1月开封市结核病防治所诊治的肺结核病人136例为肺结核组;并选择同时间段内同一医院142例健康体检者为健康对照组。比较两组一般临床资料;以实时荧光定量PCR(qRT-PCR)法检测两组血清miR-361-5p水平;检测血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)水平;分析肺结核病人血清miR-361-5p水平与VEGF、TNF-α、IFN-γ的关系;回归分析肺结核发生的影响因素;分析血清miR-361-5p对肺结核的诊断价值。结果肺结核组血清miR-361-5p[(1.58±0.45)比(1.06±0.31)]、VEGF[(389.39±46.85)pg/mL比(327.62±41.76)pg/mL]、TNF-α[(22.48±5.06)ng/L比(11.36±3.17)ng/L]水平均明显高于健康对照组,IFN-γ水平[(15.58±3.36)ng/L比(26.72±4.78)ng/L]明显低于健康对照组(均P<0.05);肺结核病人血清miR-361-5p与VEGF、TNF-α水平呈正相关,与IFN-γ水平呈负相关(均P<0.05);miR-361-5p、VEGF、TNF-α是影响肺结核发生的危险因素,IFN-γ是影响肺结核发生的保护因素(均P<0.05);血清miR-361-5p水平对肺结核发生诊断的AUC为0.898,截断值为1.44,其灵敏度为83.8%,特异度为88.0%。结论肺结核病人血清miR-361-5p呈高表达,与炎症相关因子密切相关,miR-361-5p可能与炎症相关因子相互作用,进而共同调节肺结核发生、发展。
Objective To explore the level and significance of microRNA361-5p(miR-361-5p)in serum of patients with pulmonary tuberculosis.Methods 136 cases of pulmonary tuberculosis patients treated in Kaifeng Tuberculosis Prevention and Treatment Center from March 2017 to January 2019 were analyzed and studied,and they were called pulmonary tuberculosis group;in the same period,142 healthy persons in our hospital were selected as the control group.The general clinical data of the two groups were compared and analyzed;the level of serum miR-361-5p was detected by real-time fluorescence quantitative PCR(qRT-PCR);the levels of vascular endothelial growth factor(VEGF),tumor necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)were measured;the relationships between serum level of miR-361-5p and levels of VEGF,TNF-αand IFN-γin patients with pulmonary tuberculosis were analyzed;the influencing factors of pulmonary tuberculosis were analyzed by regression analysis;and the diagnostic value of serum miR-361-5p in pulmonary tuberculosis was analyzed.Results The levels of serum miR-361-5p[(1.58±0.45)vs.(1.06±0.31)],VEGF[(389.39±46.85)pg/mL vs.(327.62±41.76)pg/mL]and TNF-α[(22.48±5.06)ng/L vs.(11.36±3.17)ng/L]in tuberculosis group were significantly higher than those in healthy group,and the level of IFN-γ[(15.58±3.36)ng/L vs.(26.72±4.78)ng/L]in tuberculosis group was significantly lower than that in healthy group(P<0.05);the serum level of miR-361-5p was positively correlated with levels of VEGF and TNF-α,and negatively correlated with level of IFN-γin patients with pulmonary tuberculosis(P<0.05);miR-361-5p,VEGF and TNF-αwere risk factors for tuberculosis,and IFN-γwas protective factor for tuberculosis(all P<0.05);the AUC of serum miR-361-5p level for diagnosis of pulmonary tuberculosis was 0.898,the truncation value was 1.44,the sensitivity was 83.8%,and the specificity was 88.0%.Conclusion The expression of serum miR-361-5p in patients with pulmonary tuberculosis is high,which is closely related to inflammatory factors,mid-361-5p may interact with inflammatory factors,and then co-regulate the occurrence and development of pulmonary tuberculosis.
作者
贾宗岭
单富强
葛国昌
赵幸娜
JIA Zongling;SHAN Fuqiang;GE Guochang;ZHAO Xinna(Respiratory Medicine,Kaifeng Tuberculosis Prevention and Treatment Center(Kaifeng Pulmonary Hospital),Kaifeng,Henan 475000,China;Zhengzhou University,Zhengzhou,Henan 450007,China)
出处
《安徽医药》
CAS
2021年第8期1609-1612,共4页
Anhui Medical and Pharmaceutical Journal
基金
河南省科技攻关项目(182102310153)。
