摘要
目的通过研究HSP70基因多态性,分析钨矿工人患矽肺的易感基因及敏感指标,服务矽肺的防控工作。方法采用病例对照研究方法,选择赣南医学院第一附属医院呼吸内科及赣州于都铁山垅镇卫生院就诊或健康检查的钨矿矽肺患者55人为矽肺组,有钨矿工作史且胸片正常的健康工人42人为对照组。所有对象于清晨空腹时抽取外周静脉血5 ml,采用PCR直接测序方法对HSP70基因多态性进行分析,并进行统计分析。结果HSP70-1190位点的3种基因型GG、GC、CC在赣南钨矿健康工人及矽肺患者之间的分布及G和C等位基因频率在两组间的分布,差异均有统计学意义(P<0.05)。HSP70-21267位点及HSP70-hom 2437位点的基因型及等位基因频率组间分布差异无统计学意义(P>0.05)。采用二元logistic回归分析矽肺影响因素时表明,经调整年龄、工龄、吸烟混杂因素后HSP70-1190位点的CC基因型发生矽肺的风险是GG基因型的的5.15倍(调整OR:5.15,95%CI:1.12~23.75)(P<0.05)。结论HSP70-1190位点CC基因型可能是钨矿工人患矽肺的易感基因。
Objective To search for the susceptibility genes and sensitive indicators of silicosis in tungsten miners through testing heat-shock protein 70 gene polymorphisms,and to serve the prevention and control of silicosis.Methods 55 tungsten mine silicosis patients were selected as the silicosis group and 44 healthy workers with history of tungsten were recruited as control group.All the subjects were selected from the Department of Respiratory Medicine of First Affiliated Hospital of Gannan Medical University and Yudu Tieshan Town Health Center in Ganzhou for medical or physical examination.In the morning,5 ml of peripheral venous blood was taken from all subjects when they were fasting,and the polymorphism of HSP70 gene was analyzed by PCR direct sequencing.Results The distribution of GG,GC,CC of HSP70-1190 locus in the healthy workers and the silicosis patients in Gannan tungsten mine was statistically significant(P<0.05).The distribution of G and C allele frequency between the two groups was also statistically significant(P<0.05).The distribution of genotype and allele frequency of HSP70-21267 and HSP70-hom 2437 between the two groups was not statistically significant(P>0.05).The risk of silicosis in CC genotype of hsp70-1190 locus was 5.15 times higher than that in GG genotype(adjusted or:5.15,95%CI:1.12-23.75)(P<0.05).Conclusion The CC genotype of HSP70-1190 may be the susceptible gene of silicosis in tungsten miners.
作者
朱涟敏
赖青云
袁小亮
陈青风
李杰
李辉
刘惟优
ZHU Lian-min;LAI Qing-yun;YUAN Xiao-liang;CHEN Qing-feng;LI Jie;LI Hui;LIU Wei-you(Pneumology department,First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,China;不详)
出处
《工业卫生与职业病》
CAS
2021年第4期308-311,317,共5页
Industrial Health and Occupational Diseases
基金
江西省卫生计生委科技项目(20175344)。
