摘要
为解决口服酶制剂易被胃液的强酸环境破坏其效价,影响乳糖不耐受症治疗效果的问题,制备一种可以保护乳糖酶过胃而不失去活性的新型植物基载体。采用新型植物基材料向日葵孢粉素外壁胶囊为载体,用于乳糖酶的负载及保护。通过负载、包膜、释放等实验以及采用扫描电子显微镜、激光共聚焦显微镜等表征手段,确定5%海藻酸钠与6%羧甲基茯苓多糖在体积比为1∶1混合所得的凝胶球体外释放效果最佳,实现在消化道中的响应性释放。进一步实验发现,经冻干处理后该体系也具备成为乳糖酶冻干保护剂的潜力。基于此,实验成功论证了向日葵孢粉素外壁胶囊递送体系可以作为乳糖酶的高效递送及保护载体的可行性,为乳糖不耐受症的治疗提供了新的思路。
The potency of traditional oral enzyme preparations for treating lactose intolerance can be readily impaired by the strong acidic environment in the stomach.In order to overcome this problem,sunflower sporopollenin exine capsules(SECs),a new plant-based carrier that can protect lactase from losing its activity,were prepared for use as a carrier for loading and protection of lactase.Loading,encapsulation and release experiments and observation by scanning electron microscopy(SEM)and laser confocal microscopy(LCM)showed that gel microspheres of sodium alginate(5%)and carboxymethylpachymaran(6%)at a mixing ratio of 1:1(V/V)gave the best release performance in vitro,allowing responsive release of lactase in simulated digestive fluids.The following experiments illustrated that this system also showed the potential as a freeze-drying protective agent for lactase.Based on this,this study successfully demonstrated the feasibility of SECs as an efficient delivery and protection carrier for lactase,which will provide a new idea for the treatment of lactose intolerance.
作者
侯欣尧
邓紫玙
梁宏闪
李斌
HOU Xinyao;DENG Ziyu;LIANG Hongshan;LI Bin(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Environment Correlative Dietology,Ministry of Education,Wuhan 430070,China;Functional Food Engineering&Technology Research Center of Hubei Province,Wuhan 430068,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第14期137-143,共7页
Food Science
基金
湖北省大学生创新创业训练计划项目(S201910504068)
中央高校基本科研业务费专项(2662018QD016)。
关键词
乳糖不耐受症
乳糖酶
孢粉素外壁胶囊
递送
保护
lactose intolerance
lactase
sporopollenin exine capsules
delivery
protection
