摘要
以N-甲基-N-亚硝基脲(MNU)为诱变剂,用半致死剂量处理燕麦品种:爱沃、陇燕4号和贝勒2代,并对获得的M_(2)代突变体进行变异与光合特性分析。结果表明,MNU处理可产生丰富的变异类型,M_(2)代突变体中以叶部变异株最多,占总变异株的46.01%;其次是株高变异株,占15.96%。MNU对不同燕麦品种的诱变效应不同,具体表现为:爱沃变异株中叶部变异株数占总数的87.76%,在株高变异株中占比67.65%,在穗部变异株数中占比64.00%,诱变效应最佳;陇燕4号的效应最低,各类型的变异株数均少于3株。而变异类型植株在灌浆期的主要光合特性如下:除叶缘黄化株外,其余变异株的叶绿素相对含量均高于对照;爱沃和贝勒2代高秆株的净光合速率较对照分别增加了52.54%和35.81%;不同变异株的潜在光化学速率、光化学量子产量和表观光合电子传递速率均高于对照,非光化学淬灭系数较对照显著下降。综上所述,除叶开裂株和叶缘黄化株外,其余变异类型的植株较对照的光合性能有所提高。
In this study,three varieties("Everleaf","Longyan No.4"and"Baler 2")of oat were treated with half lethal dose with N-methyl-N-nitrosourea(MNU).The variation and photosynthetic characteristics of obtained M_(2)generation mutants were analyzed.The results showed that MNU treatment induced multiple variant types in M_(2)generation,in which leaf mutants and plant height mutants were the dominant types,accounting for 46.01%and 15.96%,respectively.MNU had different mutagenic effects on different oat varieties,which was characterized by several aspects.The number of leaf mutants in"Everleaf"accounted for 87.76%of total mutants,67.65%of the total plant height mutants,and 64.00%of the total panicle mutants,which had the greatest mutagenic effect.While"Longyan No.4"had the lowest mutagenic effect that less than three mutants were obtained from each variant type.The main photosynthetic characteristics of the mutants at grain filling stage were shown as following aspects.The relative content of chlorophyll of mutants was higher than that of the wild type,except for the mutants with yellow leaf edge.Compared with the wild type,the net photosynthetic rate of taller mutants of"Everleaf"and"Baler 2"increased by 52.54%and 35.81%,respectively.Compared with the wild type,the mutants had higher potential photochemical rate,photochemical quantum yield,and apparent photosynthetic electron transfer rate,lower non-photochemical quenching coefficient.In summary,most mutants had higher photosynthetic performance than wild type except for leaf cracking and yellowed leaf edge mutants.
作者
李娟宁
赵桂琴
柴继宽
王苗苗
贾志锋
LI Juan-ning;ZHAO Gui-qin;CHAI Ji-kuan;WANG Miao-miao;JIA Zhi-feng(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecology System,Ministry of Education/Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China;Academy of Animal Sciences and Veterinary Medicine,Qinghai University,Xining 810016,China)
出处
《中国草地学报》
CSCD
北大核心
2021年第7期12-20,共9页
Chinese Journal of Grassland
基金
甘肃省重大专项(19ZD2NA002-3)
青海省重点实验室项目(2020-ZJ-Y03)资助。
