摘要
目的探讨荧光PCR熔解曲线法对痰样本的利福平和异烟肼相关耐药基因的检测效能。方法回顾性分析2019年7月—2020年7月沈阳市第十人民医院收取的254例痰样本检验结果,以BACTEC MGIT 960液体药敏(以下简称MGIT 960药敏)为参考标准,评价熔解曲线法检测利福平、异烟肼耐药性的效能。结果与MGIT 960药敏结果比较,利福平耐药检测的敏感性、特异性和Kappa值分别为89.6%、95.6%和0.83;异烟肼耐药检测的敏感性、特异性和Kappa值分别为81.5%、98.9%和0.85。在初治患者中具有较好的一致性,优于复治患者。结论应用熔解曲线法检测患者痰样本的利福平和异烟肼相关耐药基因,与MGIT 960药敏法比较具有较好的敏感度、特异度和一致性,可快速检测出患者的耐药情况和耐多药结核病(MDR-TB)患者,为临床患者尤其是初治患者提供快速有效的化疗参考方案。
Objective To investigate the detection efficiency of Rifampicin and Isoniazid related drug resistance genes in sputum samples by fluorescence PCR melting curve analysis.Methods The sputum test results of 254 cases in the 10th People′s Hospital of Shenyang City from July 2019 to July 2020 were retrospectively analyzed.Using BACTEC MGIT 960 liquid drug sensitivity(hereinafter referred to as MGIT 960 drug sensitivity)as the reference standard,the effectiveness of the fusion curve method in the detection of Rifampicin and Isoniazid resistance were evaluated.Results Compared with MGIT 960,the sensitivity,specificity and Kappa values of Rifampicin resistance were 89.6%,95.6%and 0.83.The sensitivity,specificity and Kappa values of Isoniazid resistance were 81.5%,98.9%and 0.85.In the initial treatment of patients with good consistency,better than the retreatment of patients.Conclusion The method to detect patients with sputum samples of Rifampicin and Isoniazid resistance genes,compared with MGIT 960 DST method has good sensitivity and consistency,specific degree,can quickly detect patients with drug resistance situation and multidrug-resistant tuberculosis(MDR-TB)patients,especially for clinical patients to treat patients with early reference provides a quick and effective chemotherapy scheme.
作者
王悦
孟湃喆
于艳红
WANG Yue;MENG Pai-zhe;YU Yan-hong(Tuberculosis Laboratory,the 10th People′s Hospital of Shenyang City,Liaoning Province,Shenyang110044,China;Medical Laboratory Technology,Shenyang Medical College,Liaoning Province,Shenyang110034,China)
出处
《中国当代医药》
CAS
2021年第20期194-197,共4页
China Modern Medicine
