摘要
目的:探讨颈动脉体化学损伤对慢性阻塞性肺疾病(COPD)大鼠呼吸运动的影响。方法:根据随机数字表法,将30只Wistar大鼠分为两组,COPD组20只:用烟熏联合LPS气道内注药法建立大鼠COPD模型;对照组10只:同等条件饲养但不进行烟熏,气道内滴注生理盐水。实验组8周造模成功后,颈部正中切开暴露双侧颈动脉体(CB),用双氧水浸润的滤纸条包裹颈总动脉分叉部,造成CB化学损伤;对照组在用同样方法分离暴露双侧颈总动脉后,用NS滤纸条包裹,对CB进行假损伤处理。分别记录每组大鼠呼吸频率、呼气间期、吸气间期、吸呼比等呼吸生理指标及血气分析结果,并进行统计学分析。造模结束后取所有大鼠双侧CB行病理学观察。结果:呼吸生理指标:CB损伤前COPD组呼吸频率明显高于对照组(t=3.186,P=0.004),CB损伤后COPD组呼吸频率更低(t=-3.266,P=0.003)、吸气时间更长(t=3.989,P<0.001)、吸呼比更高(t=6.103,P<0.001)。损伤CB后,无论是COPD组还是对照组的呼吸频率都较损伤前降低(对照组t_(1)=5.136,COPD组t_(2)=9.656,P<0.001)。血气分析结果:CB损伤前、后COPD组血浆pH、动脉氧分压均低于对照组,二氧化碳分压均高于对照组(损伤前的组间对照tpH=-5.996,t_(PaO_(2))=11.640,t_(PaO_(2))=-3.415,损伤后的组间对照t_(pH)=-13.874,t_(PaCO_(2))=13.475,t_(PaO_(2))=-17.295;损伤前、后的COPD组自身对照t_(pH)=-13.781,t_(PaCO_(2))=4.341,t_(PaO_(2))=6.019,损伤前、后的对照组自身对照t_(pH)=3.766,t_(PaCO_(2))=-2.858,t_(PaO_(2))=17.394,均P<0.05)。CB病理切片镜下可见实质细胞(球细胞和支持细胞)明显增生、体积增大、毛细血管增生。结论:CB在COPD大鼠的呼吸调控功能中起到重要作用,COPD环境下CB肥大增生从而代偿,通过增加呼吸频率提高机体通气量对抗慢性持续低氧环境。
Objective:To investigate the effect of carotid body chemical injury on respiratory movement in rats with chronic obstructive pulmonary disease(COPD).Methods:According to the method of random number table,30 Wistar rats were divided into two groups:the COPD group(n=20)and the control group(n=10).The COPD model was established by fumigation combined with LPS intratracheal injection,and the control group(n=10)was fed without fumigation under the same conditions and intratracheal instillation of normal saline.In the experimental group,after 8 weeks of successful modeling,the bilateral carotid body(CB)was exposed by median cervical incision,and the bifurcation of the common carotid artery was wrapped with a filter strip infiltrated with hydrogen peroxide,resulting in chemical injury of the common carotid body,while in the control group,after the bilateral common carotid artery was separated and exposed with the same method,the bilateral common carotid artery was wrapped with NS filter paper,and the carotid body was treated with sham injury.The respiratory physiological indexes such as respiratory frequency,expiratory interval,inspiratory interval,inspiratory/expiratory ratio and the results of blood gas analysis in each group were recorded and statistically analyzed.After the establishment of the model,the bilateral carotid arteries of all rats were taken for pathological observation.Results:Respiratory physiological indexes:the respiratory rate of COPD rats before CB injury was significantly higher than that of the control group(t=3.186,P=0.004).After CB injury,the respiratory rate of COPD rats was lower(t=-3.266,P=0.003),the inspiratory time was longer(t=3.989,P<0.001)and the inspiratory/expiratory ratio was higher than that of the control group(t=6.103,P<0.001).Self-control showed that the respiratory rate decreased in both COPD rats and control rats after CB injury(control group t_(1)=5.136,COPD group t_(2)=9.656,P<0.001).Results of blood gas analysis:plasma pH and arterial oxygen partial pressure of COPD rats before and after CB injury were lower than those of control group,while partial pressure of carbon dioxide were higher than those of control group(inter-group control before injury t_(pH)=-5.996,t_(PaCO_(2))=11.640,t_(PaO_(2))=-3.415,inter-group control after injury t_(pH)=-13.874,t_(PaCO_(2))=13.475,t_(PaO_(2))=-17.295;self-control of COPD group before and after injury t_(pH)=-13.781,t_(PaCO_(2))=4.341,t_(PaO_(2))=6.019;self-control of control group before and after injury tpH=3.766,t_(PaCO_(2))=-2.858,t_(PaO_(2))=17.394,all P<0.05).Under the pathological section of the carotid body,the parenchyma cells(glomus and sustentacular cells)were obviously proliferated,enlarged and capillaries proliferated.Conclusion:The carotid body plays an important role in the respiratory regulation of COPD rats.The hypertrophy and proliferation of the carotid body in the COPD environment can be compensated by increasing the respiratory frequency and body ventilation to resist the chronic and persistent hypoxia environment.
作者
徐北政
赵晓赟
杨洋
王菁
刘珊
XU Bei-zheng;ZHAO Xiao-yun;YANG Yang;WANG Jing;LIU Shan(School of Basic Medical Sciences,Tianjin Medical University,Tianjin 300070,China;Clinical College of Thoracic Medicine,Tianjin Medical University,Tianjin 300222,China;Department of Pulmonary and Critical Care Medicine,Tianjin Chest Hospital,Tianjin 300222,China;Department of Endocrinology,Tianjin Chest Hospital,Tianjin 300222,China;Department of Pathology,Tianjin Chest Hospital,Tianjin 300222,China;Tianjin Institute of Cardiovascular Disease,Tianjin 300222,China)
出处
《天津医科大学学报》
2021年第4期349-353,共5页
Journal of Tianjin Medical University
基金
国家重点研发计划(2019YFC0119404)
天津市卫生行业重点攻关项目(15KG129)
天津医科大学大学生创新创业资助计划项目(201910062078)。
关键词
慢性阻塞性肺疾病
大鼠模型
CB
化学感受器
呼吸调控
chronicobstructive pulmonary disease
rat model
carotid body
chemoreceptor
respiratory regulation
