摘要
目的:研究维生素C对酒精性肝损伤大鼠肠道菌群的影响。方法:52只雄性SD大鼠随机分为正常对照组、酒精模型组及维生素C低、中、高剂量干预组(维生素C剂量分别为20 mg·kg^(-1)·d^(-1)、100 mg·kg^(-1)·d^(-1)、500 mg·kg^(-1)·d^(-1))。以梯度酒精灌胃的方法连续饲养6周复制酒精性肝损伤模型,正常对照组和酒精模型组给予蒸馏水灌胃,其余组予以维生素C进行干预。末次灌胃后采血,肝脏称重,计算肝体比,检测大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活力、超氧化物歧化酶(SOD)和丙二醛(MDA);对大鼠粪便中的需氧细菌、乳酸杆菌、双歧杆菌、大肠杆菌和肠球菌进行平板培养并计数。结果:维生素C能够抑制酒精引起的ALT、AST、MDA、肝体比升高和SOD降低(P<0.05),抑制酒精引起的肠道需氧菌、大肠杆菌、肠球菌数量增多和乳酸杆菌、双歧杆菌数量减少(P<0.05),且呈现一定剂量反应关系。结论:维生素C可有效降低大鼠血清转氨酶,调节机体抗氧化失衡,改善酒精引起的大鼠肝脏损伤,其作用机制可能是:维生素C通过调节肠道菌群结构,增加有益菌(如乳酸杆菌、双歧杆菌)数量,减少有害菌(如需氧菌、大肠杆菌、肠球菌)数量,从而改善肠道菌群紊乱,保护肠道黏膜,促进肝脏损伤的恢复。
Objective: To study the effects of vitamin C on the intestinal flora of rats with alcoholic liver injury. Methods: Fifty-two male SD rats were randomly divided into normal control group, alcohol model group, and vitamin C low, medium, and high dose intervention groups(20 mg·kg^(-1)·d^(-1), 100 mg·kg^(-1)·d^(-1), 500 mg·kg^(-1)·d^(-1)). The alcoholic liver injury model was reproduced by the method of gastric gavage with gradient alcohol for 6 weeks. The normal control group and the alcohol model group were given distilled water by gavage, the rest of the groups were given vitamin C for intervention. After the last gavage, blood was collected, the serum was separated, and the livers were weighed. The liver-to-body ratio were calculated, ALT, AST, SOD and MDA in serum of rats were detected. Aerobic bacteria, Lactobacillus, Bifidobacterium, Escherichia coli and Enterococcus were cultured in the feces of rats by plate culture and the amount of those bacteria in feces of rats was calculated. Results: Compared with the model group, the ALT, AST, MDA, liver-to-body ratio of the vitamin C intervention groups were significantly reduced and SOD was elevated(P<0.05), at the mean time, the number of intestinal aerobic bacteria, Escherichia coli, Enterococcus were decreased, Lactobacillus and Bifidobacterium were increased(P<0.05), with a certain dose response relationship. Conclusion: Vitamin C can effectively reduce rat serum transaminase, regulate the body’s antioxidant imbalance, and improve alcohol-induced liver damage in rats. One of its mechanism of action may be: Vitamin C increases the beneficial bacteria(such as Lactobacilus, Bifidobacterium), reduce the number of harmful bacteria(such as aerobic bacteria, Escherichia coli, Enterococcus), to regulate the structure of intestinal flora, improve the intestinal flora disorder, protect the intestinal mucosa, and promote the recovery of liver damage.
作者
匡逸静
谢安琪
田鹏
陶泳兵
沈奎
李永儒
杨艳
KUANG Yijing;XIE Anqi;TIAN Peng;TAO Yongbing;SHEN Kui;LI Yongru;YANG Yan(Preventive Medicine Undergraduate,School of Public Health,Southwest Medical University,Luzhou 646000,China;Department of Hygiene Inspection and Quarantine,School of Public Health,Southwest Medical University,Luzhou 646000,China;Department of Nutrition and Food Hygiene,School of Public Health,Southwest Medical University,Luzhou 646000,China)
出处
《现代食品》
2021年第10期192-195,202,共5页
Modern Food
基金
国家级大学生创新创业训练计划项目(201810632099)。
关键词
维生素C
酒精性肝损伤
肠道菌群
氧化应激
vitamin C
alcoholic liver injury
intestinal flora
oxidative stress
