摘要
为探索GPRIN3基因的生物学特性,以麦洼牦牛为试验材料,利用RT-PCR技术扩增GPRIN3基因编码区序列并对其进行生物信息学分析,采用实时荧光定量PCR技术检测GPRIN3在牦牛不同组织的mRNA表达水平.结果表明,克隆得到GPRIN3基因编码区序列大小为2343 bp,编码780个氨基酸残基;麦洼牦牛与野牦牛氨基酸序列相比,一致性为99.62%,共有3个位点发生突变,分别是103(G→R)、366(T→M)、404(E→K).系统进化分析显示,牦牛与野牦牛亲缘关系最近,其次是普通牛,与黑猩猩的亲缘关系最远;含有GRIN-C超家族保守功能结构域;实时荧光定量PCR结果表明,GPRIN3在麦洼牦牛肺脏组织中表达量最高,其次是肝脏,在臀肌中表达量最低.本研究为进一步探讨牦牛GPRIN3基因及其编码蛋白的结构和功能提供理论基础,为牦牛分子育种提供新思路.
In order to explore the correlation between GPRIN3 and growth and development of yak,Maiwa yak was used as the experimental material,and the coding region sequence of GPRIN3 was amplified by RT-PCR and then the sequences were analyzed using bioinformatics,the mRNA expression level of GPRIN3 in different tissues of yak were determined using real-time PCR.The results showed that the coding region sequence of GPRIN3 was 2343 bp,encoding 780 amino acids;compared with the amino acid sequence of the wild yak,the similarity was 99.62%,there were three mutations:103(G→R),366(T→M),and 404(E→K).Phylogenetic analysis showed that yak showed the closest relationship with Bosmutus,followed by Bostaurus,and distant with Pongoableii;It contained the conserved functional domain of the GRIN-C superfamily;The real-time quantitative PCR results showed that GPRIN3 had the highest expression in the lung tissues of Maiwa yak,followed in the liver,and the lowest expression in gluteal muscle.This study provides a theoretical basis for further exploring the structure and function of the yak GPRIN3 gene and its encoded protein,and provides new ideas for yak molecular breeding.
作者
向娅
柴志欣
武志娟
王吉坤
钟金城
信金伟
XIANG Ya;CHAI Zhi-xin;WU Zhi-juan;WANG Ji-kun;ZHONG Jin-cheng;XIN Jin-wei(Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization,Sichuan Province,Southwest Minzu University,Chengdu 610041,China;Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization,Ministry of Education,Southwest Minzu University,Chengdu 610041,China;State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa 85000,China)
出处
《西南民族大学学报（自然科学版）》
CAS
2021年第4期356-369,共14页
Journal of Southwest Minzu University(Natural Science Edition)
基金
西藏自治区科技重点研发及转化项目(XZ202001ZY0016N)
西南民族大学青藏高原生态畜牧业协同创新中心项目(2020PTJS22002)
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-37)。
