摘要
目的探讨胃癌组织中叉头框蛋白P1(FOXP1)和叉头框蛋白Q1(FOXQ1)的表达,并分析其与临床病理特征及预后关系。方法选取2014年1月~2017年2月在宜宾市中医医院普外科确诊并接受根治性手术的胃癌患者156例为研究对象,采用免疫组化EnVision法分别检测患者胃癌组织及癌旁组织中FOXP1和FOXQ1的表达,分析其与临床病理参数的关系;采用Spearman相关性分析胃癌组织中FOXP1和FOXQ1表达的相关性;采用Cox比例风险回归模型分析患者预后的独立危险因素;采用Kaplan-Meier生存分析法分析FOXP1,FOXQ1表达与总体生存时间(OS)的关系。结果胃癌组织中FOXP1高表达率低于癌旁组织,FOXQ1高表达率高于癌旁组织,差异均有统计学意义(χ^(2)=23.521,35.193,均P<0.05);胃癌组织中FOXP1,FOXQ1的表达与病理分化程度、TNM分期、淋巴结转移、肿瘤大小、脉管神经侵犯和浸润深度相关(χ^(2)=6.054~14.909,均P<0.05);Spearman相关分析显示,胃癌组织中FOXP1与FOXQ1表达呈负相关(r=-0.526,P<0.05);多因素Cox回归分析显示,FOXP1低表达、FOXQ1高表达、FOXP1低或无表达/FOXQ1高表达均是影响患者OS的独立危险因素(P<0.05);Kaplan‐Meier法分析结果显示,FOXP1低或无表达/FOXQ1高表达患者OS短于其他患者(χ^(2)=57.228,P<0.05);FOXP1低或无表达/FOXQ1高表达组OS短于FOXP1低或无表达患者和FOXQ1高表达患者(χ^(2)=8.112,P<0.05)。结论FOXP1蛋白在胃癌组织中低表达,FOXQ1蛋白在胃癌组织中高表达,两者与疾病的发生发展及预后密切相关,两者联合检测能更好地判断患者的预后。
Objective To explore the expression of FOXP1 protein and FOXQ1 protein in gastric cancer,and analyze their relationship with clinicopathological features and prognosis.Methods 156 cases of gastric cancer patients who were diagnosed and underwent radical operation in Department of General Surgery of the Traditional Chinese Medicine Hospital of Yibin from January 2014 to February 2017 were selected as the research objects.EnVision immunohistochemical method was used to detect the expression of FOXP1 and foxq1 in gastric cancer tissues and adjacent normal tissues.The expression levels of FOXP1 and FOXQ1 in gastric cancer and their relationship with clinicopathological parameters were analyzed.Spearman correlation was used to analyze the correlation between FOXP1 and FOXQ1 in gastric cancer.Cox proportional hazards regression model was used to analyze the risk factors for the prognosis of gastric cancer patients.Kaplan-Meier survival analysis was used to analyze the relationship between FOXP1,FOXQ1 expression and overall survival time(OS).Results The high expression rate of FOXP1 in gastric cancer tissues was lower than that in adjacent normal tissues,and the high expression rate of FOXQ1 was higher than that in adjacent normal tissues,the difference were statistically significant(χ^(2)=23.521,35.193,all P<0.05).The expression of FOXP1 and FOXQ1 in gastric cancer was correlated with pathological differentiation,TNM stage,lymph node metastasis,tumor size,vascular and nerve invasion and depth of invasion(χ^(2)=6.054~14.909,all P<0.05).Spearman correlation analysis showed that FOXP1 expression was negatively correlated with FOXQ1 expression in gastric cancer(r=-0.526,P<0.05).Multivariate Cox regression analysis showed that low or no expression of FOXP1,high expression of FOXQ1,FOXP1low or no expression/FOXQ1high expression were independent risk factors for overall survival(OS)of gastric cancer patients(P<0.05).Kaplan-Meier analysis showed that the OS of patients with FOXP1low or no expression/FOXQ1 high expression was shorter than that of other patients(χ^(2)=57.228,P<0.05).The OS of patients with FOXP1low or no expression/FOXQ1high expression was shorter than that of patients with low or no expression of FOXP1 or high expression of FOXQ1(χ^(2)=8.112,P<0.05).Conclusion FOXP1 protein was low expression in gastric cancer tissue,FOXQ1 protein was high expression in gastric cancer tissue,both were closely related to the occurrence,development and prognosis of the disease,combined detection of the two can better judge the prognosis of patients.
作者
朱涛
王伟
吴和刚
ZHU Tao;WANG Wei;WU He-gang(Department of General Surgery,the Traditional Chinese Medicine Hospital of Yibin,Sichuan Yibin 644000,China;Department of General surgery,the First People’s Hospital of Yibin,Sichuan Yibin 644000,China;Department of Pathology,the First People’s Hospital of Yibin,Sichuan Yibin 644000,China)
出处
《现代检验医学杂志》
CAS
2021年第4期68-73,共6页
Journal of Modern Laboratory Medicine
