摘要
目的:研究车前子多糖对乳腺癌细胞增殖、迁移和侵袭的影响,并初步探究其作用机制。方法:以人乳腺癌细胞MDA-MB-231为对象,采用MTT法检测不同质量浓度车前子多糖(8、16、32、64 mg/L)对细胞增殖能力的影响并计算细胞存活率;采用划痕实验、Transwell侵袭实验分别检测不同质量浓度车前子多糖(8、16 mg/L)对细胞迁移和侵袭能力的影响;采用Western blot法检测转移及上皮间质转化(EMT)相关蛋白[基质金属蛋白酶2(MMP-2)、MMP-9、上皮黏钙素(E-cadherin)、神经黏钙素(N-cadherin)、波形蛋白(vimentin)]的表达情况。结果:MTT实验结果显示,车前子多糖32、64 mg/L组细胞的存活率均较对照组显著降低(P<0.05或P<0.01),故以不影响细胞存活率的8、16 mg/L作为后续实验给药浓度。与对照组比较,车前子多糖8、16 mg/L组细胞的相对迁移率(12、24 h)、相对侵袭率以及MMP-2、MMP-9、N-cadherin、vimentin蛋白的相对表达量均显著降低(P<0.05或P<0.01),E-cadherin蛋白的相对表达量均显著升高(P<0.05或P<0.01)。结论:车前子多糖能够在体外抑制乳腺癌细胞MDA-MB-231的增殖,并可能通过抑制转移及EMT相关蛋白的表达来抑制该细胞的迁移和侵袭。
OBJECTIVE:To study the effects of Plantago asiatica polysaccharide on the proliferation,migration and invasion of breast cancer cells,and to investigate its mechanism preliminarily.METHODS:Using human breast cancer cell MDA-MB-231 as subjects,MTT method was adopted to detect the effects of different concentrations of P.asiatica polysaccharide(8,16,32,64 mg/L)on the cell proliferation ability,and survival rate of the cells was calculated.Scratch test and Transwell invasion test were used to detect the effects of different concentrations of P.asiatica polysaccharide(8,16 mg/L)on cell migration ability and invasion ability.Western blot assay was used to detect the expression of epithelial-mesenchymal transition(EMT)-related proteins[matrix metalloproteinase-2(MMP-2),MMP-9,E-cadherin,N-cadherin,vimentin].RESULTS:Results of MTT assay showed that survival rate of the cells in 32,64 mg/L P.asiatica polysaccharide groups were significantly lower than control group(P<0.05 or P<0.01),so that 8,16 mg/L,which did not affect the cell survival rate,were used as the follow-up drug concentrations.Compared with control group,relative mobility(12,24 h),relative invasion rate and relative expression of MMP-2,MMP-9,N-cadherin and vimentin protein were decreased significantly in 8,16 mg/L P.asiatica polysaccharide groups(P<0.05 or P<0.01),while relative expression of E-cadherin protein was increased significantly(P<0.05 or P<0.01).CONCLUSIONS:P.asiatica polysaccharide can inhibit the proliferation of breast cancer cells MDA-MB-231,and inhibit the migration and invasion of the cells by regulating the expression of metastasis and EMT-related proteins.
作者
陈高
杨晓婷
王曦
金湛
朱瑞
甘椿椿
周道堂
姚水洪
CHEN Gao;YANG Xiaoting;WANG Xi;JIN Zhan;ZHU Rui;GAN Chunchun;ZHOU Daotang;YAO Shuihong(School of Medicine,Quzhou College of Technology,Zhejiang Quzhou 324000,China;Dept.of Urology,Quzhou Municipal People’s Hospital,Zhejiang Quzhou 324000,China;College of Pharmacy,Zhejiang University of TCM,Hangzhou 310053,China;Dept.of Pharmacy,Kaili Municipal First People’s Hospital,Guizhou Kaili 556000,China)
出处
《中国药房》
CAS
北大核心
2021年第15期1848-1853,共6页
China Pharmacy
基金
贵州省卫生健康委科学技术基金项目(No.gzwjkj2019-1-079)
衢州市科技计划指导性项目(No.2019121)。
关键词
车前子多糖
乳腺癌
MDA-MB-231细胞
增殖
迁移
侵袭
Plantago asiatica polysaccharide
Breast cancer
MDA-MB-231 cells
Proliferation
Migration
Invasion
