摘要
采用单宁酶法从马桑皮中提取没食子酸,以单因素-响应面实验优化了提取工艺,对得到的没食子酸进行了纯化和结构鉴定,考察了纯化后没食子酸的抗氧化活性,并与传统的酸提取法相对比。结果表明,在马桑皮质量2.00 g、固液比1∶40(g∶m L)、单宁酶添加量为20 U/g(以马桑皮的质量为基准)、体系pH 5.0、提取温度48℃、提取时间4 h的条件下,没食子酸提取率可达10.79%±0.65%。经NKA-9大孔吸附树脂纯化后,酶法制备的没食子酸的纯度为96.0%,高于酸提取法制备的没食子酸纯度(81.5%)。与酸提取法相比,酶法制备的没食子酸具有更强的抗氧化活性,在质量浓度为256 mg/L时,其对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟基自由基(·OH)的清除率分别为78.81%和61.99%,对Fe^(3+)还原能力为0.427。
Tannase was used to extract gallic acid from Coriaria nepalensis bark,and the extraction process was optimized by single factor and response surface experiments.The obtained gallic acid was purified and identified.The antioxidant activity of the purified gallic acid was investigated and compared with that of the sample extracted by traditional acid extraction method.The results showed that when Coriaria nepalensis bark mass was 2.00 g,solid liquid ratio was 1∶40(g∶m L),tannase addition amount was 20 U/g(based on the mass of Coriaria nepalensis bark),pH of the system was 5.0,extraction temperature and extraction time was 48℃ and 4 h,the extraction yield of gallic acid could reach 10.79%±0.65%.After purification by NKA-9 macroporous adsorption resin,the purity of gallic acid prepared by enzymatic method was 96.0%,which was higher than that of gallic acid(81.5%)prepared by acid extraction method.Compared with that prepared by acid extraction method,gallic acid prepared by enzymatic method had stronger antioxidant activity.The scavenging rate of 1,1-diphenyl-2-picrylhydrazyl radicals(DPPH·)and hydroxyl radicals(·OH)by gallic acid with a mass concentration of 256 mg/L were 78.81% and 61.99%,respectively,and the reduction ability of Fe^(3+) was 0.427.
作者
郭林新
强涛涛
马养民
任龙芳
GUO Linxin;QIANG Taotao;MA Yangmin;REN Longfang(College of Bioresources Chemical and Materials Engineering,Shaanxi University of Science&Technology,Xi'an 710021,Shaanxi,China;College of Chemistry and Chemical Engineering,Ankang University,Ankang 725000,Shaanxi,China;Key Laboratory of Chemical Additives for China National Light Industry,Xi'an 710021,Shaanxi,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2021年第7期1423-1429,共7页
Fine Chemicals
基金
陕西省重点科技创新团队(2020TD-009)
陕西省教育厅重点科学研究计划(协同创新中心项目)(20JY003)。
关键词
响应面
单宁酶
马桑皮
没食子酸
提取技术
response surface
tannase
Coriaria nepalensis bark
gallic acid
extraction technology
