摘要
目的探讨miR-21、miR-218和miR-23a的表达水平对新辅助化疗乳腺癌患者预后评价的临床意义。方法选取2016年6月至2017年3月间上海市金山区亭林医院诊断并治疗的60例乳腺癌患者进行前瞻性研究。入组后分别取乳腺癌组织以及正常乳腺组织,根据3年随访生存情况分为生存组32例和死亡组28例。分别对乳腺癌组织以及正常乳腺组织中、生存组以及死亡组患者的miR-21、miR-218及miR-23a的表达水平进行比较。探讨miR-21、miR-218及miR-23a表达水平的联合检测对死亡患者的预测效能。结果癌组织的miR-21和miR-23a高于癌旁组织,癌组织的miR-218低于癌旁组织;生存组患者的miR-21和miR-23a低于死亡组,生存组患者的miR-218高于死亡组,差异均有统计学意义(均P<0.05)。miR-21、miR-218和miR-23a联合检测对死亡患者预测特异度优于单独检测;通过对患者死亡情况的受试者工作特征(ROC)曲线分析,联合检测的曲线下面积高于单独检测,miR-21、miR-218和miR-23a的临界值分别为5.55、0.99和1.83。结论miR-21、miR-218和miR-23a的表达水平对新辅助化疗乳腺癌患者预后评价具有重要价值,未来可作为患者预后评价及靶向治疗的重要参考。
Objective To study the clinical significance of the expression levels of miR-21,miR-218 and miR-23 a in evaluating the prognosis of breast cancer patients on neoadjuvant chemotherapy.Methods In this study,60 breast cancer patients diagnosed and treated at Tinglin Hospital of Jinshan District from June 2016 to March 2017 were selected.Normal breast tissue and breast cancer tissue samples were obtained from the participants.After a follow-up of 3 years,they were categorized into a survival group(32 patients)and a death group(28 patients).The expressions of miR-21,miR-218 and miR-23 a were compared among breast cancer tissues,normal breast tissues,survival group and death group.The predictive effect of combination detection of miR-21,miR-218 and miR-23 a expression levels for death was discussed.Results MiR-21 and miR-23 a levels were significantly higher in cancer tissue than in paracancerous tissues,and miR-218 levels were lower in cancer tissues than in paracancerous tissues.The levels of miR-21 and miR-23 a were significantly lower in the survival group than in the death group,and the levels of miR-218 were significantly higher in the survival group than in the death group.The specificity of combination detection of miR-21,miR-218 and miR-23 a was significantly better than that of single detection.The area under the curve was significantly higher for the combination detection than for the single detection,and the threshold value was 5.55,0.99 and 1.83,respectively for miR-21,miR-218 and miR-23 a.Conclusion The expression levels of miR-21,miR-218 and miR-23 a were important for the prediction of prognosis of breast cancer patients on neoadjuvant chemotherapy,and can be used as an important reference for prognosis evaluation and targeted therapy in the future.
作者
吴春锋
夏训和
沈晓燕
田发
WU Chun-feng;XIA Xun-he;SHEN Xiao-yan;TIAN fa(Department of Laboratory,Tinglin Hospital of Jinshan District,Shanghai 201505,China)
出处
《中国肿瘤临床与康复》
2021年第7期778-781,共4页
Chinese Journal of Clinical Oncology and Rehabilitation
