摘要
枯草芽孢杆菌(Bacillus subtilis)作为一种重要的原核表达宿主菌,具有蛋白分泌能力强、遗传背景清晰、无密码子偏好性、生长迅速和非致病性等优点,一直都是外源蛋白表达优选的模式菌株。信号肽(signal peptide)是一段存在于前体蛋白N-端的短肽链,其功能在于引导和调节前体蛋白的折叠,在蛋白转移和分泌过程中扮演着极其重要的角色。目前,利用枯草芽孢杆菌信号肽,针对不同外源蛋白的高效分泌暂无规律性可寻。为此,从枯草芽孢杆菌信号肽的结构特点、分类、转运途径和应用方式等方面进行综述,以期为进一步筛选外源蛋白在枯草芽孢杆菌表达系统中的最适信号肽提供一定的参考。
As an important prokaryotic expression host strain,Bacillus subtilis has always been regarded as preferred model strain for foreign protein expression,featuring strong protein secretion,clear genetic background,no codon preference,rapid growth and nonpathogenicity.Signal peptide is a short peptide chain located in the N-terminal of the precursor protein,with the function of guiding and regulating the folding of the precursor protein.Meanwhile,it plays a very important role in the process of protein transfer and secretion.At present,there is no regularity to find the efficient secretion of different foreign proteins by using the signal peptide of B.subtilis.For this reason,the structural characteristics,classification,transport pathway and application of signal peptides from B.subtilis are reviewed here,aiming to provide certain reference for further screening the optimal signal peptides of foreign proteins in B.subtilis expression system.
作者
苗华彪
曹艳
杨梦瀚
黄遵锡
MIAO Hua-biao;CAO Yan;YANG Meng-han;HUANG Zun-xi(College of Life Sciences,Yunnan Normal University,Kunming 650500;Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,Kunming 650500)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第6期259-271,共13页
Biotechnology Bulletin
基金
国家自然科学基金项目(31660304)
国家重点研发计划(2017YFB0308401)。
关键词
枯草芽孢杆菌
信号肽
分泌途径
信号肽文库
Bacillus subtilis
signal peptide
secretion pathway
signal peptide library
