摘要
为探究黄果厚壳桂的耐盐能力,采用水培方法,用不同浓度的NaCl(0、50、100、150 mmol/L)处理1年生幼苗,通过测定叶片的相关生理指标,分析不同浓度盐胁迫下黄果厚壳桂的活性氧代谢、叶绿素含量和叶绿素荧光特性的响应变化。结果表明,盐胁迫处理的黄果厚壳桂叶片中MDA含量显著升高,且随着盐浓度的升高而增加;SOD、POD活性出现先增后降的趋势;叶绿素含量、Fm、Fv/Fm呈下降趋势,而叶片荧光参数Fo、NPQ呈上升趋势。可见,黄果厚壳桂对低浓度(50 mmol/L)盐胁迫表现出一定的抗性,但当NaCl浓度增大时(100 mmol/L),其细胞膜结构及叶绿体结构的受损程度较重。
In this study,1 year old seedlings of Cryptocarya concinna were treated with 1/2 Hoagland culture medium containing different NaCl concentrations(0,50,100,150 mmol/L)to explore the salt tolerance.The physiological characteristics in the leaves were determined,and then analyzed the changes of reactive oxygen metabolism,chlorophyll content and fluorescence characteristics of Cryptocarya concinna seedlings under different salt stress.The results showed the MDA content under salt treatment was higher than the control and positive correlation with NaCl concentration.The SOD and POD enzyme activities in the leaves increased first and then decreased.Compared to the control,the chlorophyll content,Fm and Fv/Fm decreased,and Fo and NPQ increased under salt stress.Overall,Cryptocarya concinna showed certain resistance to 50 mmol/L low concentration salt stress,but when NaCl concentration increased to 100 mmol/L,the damage degree of cell membrane structure and chloroplast mechanism was more serious.
作者
李俊贞
何乐祖
赵春梅
覃永华
黄松殿
刘世男
LI Junzhen;HE Lezhu;ZHAO Chunmei;QIN Yonghua;HUANG Songdian;LIU Shinan(Nanning Arboreturn of Guangxi,Nanning 530004,China;Guangxi Forestry Inventory and Planning Institute,Nanning 530004,China;College of Forestry,Guangxi University,Nanning 530004,China)
出处
《山西农业科学》
2021年第8期919-923,共5页
Journal of Shanxi Agricultural Sciences
基金
广西林业科技推广示范项目(科学研究与技术攻关类)(桂林科研[2021]18号)。
关键词
黄果厚壳桂
盐胁迫
水培法
抗氧化酶
叶绿素荧光
Cryptocarya concinna
salt stress
water culture method
antioxidant enzymes
chlorophyll fluorescence
