摘要
以薏苡CDT和Y 159种子为材料,采用不同剂量的60Co-γ及不同浓度的甲基磺酸乙酯(ethyl methylsulfone, EMS)分别对种子进行诱变处理,分析60Co-γ辐照和EMS对薏苡种子萌发和幼苗突变率、株高的影响,旨在筛选60Co-γ辐照和EMS诱变薏苡种子的最适剂量(浓度)。结果表明,60Co-γ辐照剂量和EMS浓度对薏苡种子发芽率和幼苗突变率、株高有显著影响,表现为随着60Co-γ辐照剂量和EMS浓度的增加,种子萌发时间延迟、发芽率降低和幼苗变短,而幼苗突变率逐渐增加;60Co-γ辐照处理下,薏苡CDT和Y 159的半致死剂量分别为406.305 Gy和284.795 Gy, EMS处理下薏苡Y 159的半致死剂量为2.453%。
In this paper, the seeds of Coix lacryma-jobi varieties CDT and Y 159 were treated with different doses of 60Co-γ and ethyl methylsulfone(EMS) at different concentrations respectively, and the effects of 60Co-γ irradiation and EMS on seed germination, seedling mutation rate and plant height were analyzed, which aimed to screen the optimal irradiation dose(60Co-γ) and concentration(EMS) for inducing mutation of C. lacryma-jobi. The results showed that 60Co-γ irradiation dose and EMS concentration had significant effects on seed germination rate, seedling mutation rate and plant height. With the increase of 60Co-γ irradiation dose and EMS concentration, the seed germination time was delayed, the germination rate was decreased and the seedling was shorter, while the seedling mutation rate was gradually increased. Additionally, half lethal doses for CDT and Y 159 were 406.305 Gy and 284.795 Gy under 60Co-γ irradiation respectively, while half lethal doses for Y 159 was 2.453% under EMS treatment.
作者
杨玲玲
刘凡值
孟庆杨
申刚
YANG Lingling;LIU Fanzhi;MENG Qingyang;SHEN Gang(Institute of Subtropical Crops Research,Guizhou Academy of Agricultural Sciences,Xingyi Guizhou 562400,China;School of Life Sciences,Guizhou Normal University,Guiyang 550006,China)
出处
《种子》
北大核心
2021年第7期85-89,共5页
Seed
基金
贵州省农科院青年科技基金项目“薏苡类黄酮抗氧化性及其代谢相关基因表达分析”(黔农科院青年科技基金[2021]19)
贵州省第五批人次基地建设支持经费“贵州省亚热带作物科技创新人才基地”([2016]22)。
