摘要
建立高效液相色谱测定化妆品中聚硅氧烷-15含量的方法。样品用异丙醇超声提取,以异丙醇-乙腈-水为流动相梯度洗脱,经Agilent Poroshell 120 EC-C_(18)型色谱柱(100 mm×4.6 mm,4μm)分离后,用二极管阵列检测器进行检测。以保留时间和紫外吸收光谱定性,色谱峰面积外标法定量。聚硅氧烷-15的质量浓度在3~1000 mg/L范围内线性关系良好,相关系数为0.9998,检出限为0.01%,定量限为0.03%。测定结果的相对标准偏差为2.06%(n=6),加标回收率为96.3%~101.7%。该方法快速、高效、准确,适用于化妆品中聚硅氧烷-15含量的测定。
A high performance liquid chromatographic method was established for determining polysilicone-15 in cosmetics.The samples were extracted with 2-propanol,then separated on an Agilent Poroshell 120 EC-C_(18) column(100 mm×4.6 mm,4μm)utilizing a mobile phase of 2-propanol-acetonitrile-water with gradient elution and detected by DAD.The qualitative analysis was based on retention time and ultraviolet absorption spectrum,while quantitative analysis was performed by the external standard method of chromatographic peak area.There was good linear relationship for polysilicone-15 in the mass concentration range of 3-1000 mg/L with the correlation coefficient of 0.9998.The limit of detection was 0.01%,and the limit of quantitation was 0.03%,respectively.The relative standard deviation of the detection results was 2.06%(n=6),and the recoveries were between 96.3%and 101.7%.The method is fast,efficient,accurate,and it can be applied for the determination of polysilicone-15 in cosmetics.
作者
温少楷
梁文耀
王继才
夏泽敏
谭焯针
席绍峰
郭长虹
谭建华
Wen Shaokai;Liang Wenyao;Wang Jicai;Xia Zemin;Tan Zhuozhen;Xi Shaofeng;Guo Changhong;Tan Jianhua(National Quality Supervision and Testing Center for Cosmetics(Guangzhou),Guangzhou Quality Supervision and Testing Institute,Guangzhou 511447,China)
出处
《化学分析计量》
CAS
2021年第8期55-58,共4页
Chemical Analysis And Meterage
关键词
聚硅氧烷–15
化妆品
防晒剂
高效液相色谱法
polysilicone-15
cosmetics
sun-screening agent
high performance liquid chromatography
