摘要
本研究利用农业标准(NY/T1432-2014)推荐的SSR核心引物,以云南保山推广种植的15个玉米品种为研究材料,进行遗传多样性分析。结果表明,共有17对引物在玉米品种间产生72个多态性片段,每对引物扩增出3~6个多态性片段,平均每个引物4.3个;有效等位基因数为1.219~1.750,平均为1.448;Nei's遗传多样性指数为0.176~0.418,平均为0.283;Shannon信息指数为0.315~0.605,平均为0.444;SSR多态信息量(PIC)平均值为0.686,变幅为0.512~0.812。聚类分析结果显示,遗传相似系数平均0.632,变幅为0.417~0.819;在遗传相似系数0.65处可将15个品种划分为五类。本研究可以为云南省保山玉米品种的选育和推广提供理论和技术支持。
The genetic diversity of 15 maize and it’s popularization grown in Baoshan City were carried out based on SSR primers recommended by Chinese Agricultural Industry Standard(NY/T1432-2014).The results showed that 17 pairs of SSR primers presented polymorphic,and 72 alleles were detected.The number of alleles detected by each pair of primers was 3 to 6,with an average of 4.3.The effective number of alleles was 1.219 to 1.750,with an average of 1.448.The Nei's genetic diversity index was 0.176 to 0.418,with an average of 0.283.The Shannon information index was 0.315 to 0.605,with an average of 0.444.The SSR polymorphic information(PIC)distribution ranges from 0.512 to 0.812,with an average of 0.686.The cluster analysis showed that the genetic similarity coefficient ranged from 0.417 to 0.819,with an average of 0.632,when the genetic similarity coefficient was 0.65,15 varieties were divided into 5 groups.The results can provide theoretical and technical support for breeding and promotion of Baoshan maize varieties in Yunnan Province.
作者
李松
施德林
娄灯吉
史云东
Li Song;Shi Delin;Lou Dengji;Shi Yundong(School of Chemical Biology and Environment,Yuxi Normal University,Yuxi,653100;Yuxi SeedManagement Station,Yuxi,653100)
出处
《分子植物育种》
CAS
北大核心
2021年第14期4696-4702,共7页
Molecular Plant Breeding
基金
云南省地方本科高校联合青年项目(2017FH001-099)
国家自然科学基金(31960371)共同资助。
关键词
玉米
SSR分子标记
聚类分析
遗传多样性
Maize(Zea mays L.)
SSR markers
Cluster analysis
Genetic diversity
