摘要
根据21个已知萝卜染色体组成的标准材料及其基因组中萝卜染色体的组成信息,利用SSR分子标记技术,确定了萝卜C染色体的特异SSR标记RsSA078-170和RH148-190,并应用这2个标记对甘蓝型油菜-萝卜C染色体附加系CC与普通白菜559杂交获得的F_(1)进行外源萝卜C染色体鉴定,结果表明全部F_(1)单株都扩增出了萝卜C染色体的特异条带。同时,利用萝卜基因组特异探针pURsN对F_(1)基因组进行荧光原位杂交鉴定,结果显示F_(1)有杂交信号,表明F_(1)含有萝卜基因组,该结果佐证了SSR特异标记RsSA078-170和RH148-190鉴定结果的准确性。说明SSR特异标记RsSA078-170和RH148-190可以用于甘蓝型油菜-萝卜C染色体附加系与普通白菜杂交后代中外源萝卜C染色体的鉴定。
According to 21 standard materials with known radish-chromosome information in the genetic background,two SSR molecular markers RsSA078-170 and RH148-190,which were specific for radish chromosome C,were confirmed.Both SSR markers were used to identify the alien radish chromosome C in the genetic backround of hybrid F_(1) being derived from the cross of rape-radish chromosome C addition line and pakchoi‘559’.The result showed that all individuals of F_(1) were amplified with the specific bands of radish chromosome C.Meanwhile,the radish genomic probe pURsN was used to detect the radish genomoe of F_(1) by fluorescence in situ hybridization technology.The result showed that F_(1) had hybridization signal indicating that F_(1) was added with alien radish chromosome,which proved the accuracy of the identification results of two SSR markers RsSA078-170 and RH148-190.It showed that both SSR markers could be used to identify the radish chromosome C in the progeny of rape-radish chromosome C addition line and pakchoi.
作者
丁云花
Holger Budahn
赵泓
赵岫云
DING Yunhua;Holger Budahn;ZHAO Hong;ZHAO Xiuyun(Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China;Julius Kühn-Institute,Institute for Breeding Research on Horticultural Crops,Quedlinburg D-06484,Germany)
出处
《中国蔬菜》
北大核心
2021年第8期46-50,共5页
China Vegetables
基金
国家自然科学基金项目(31672152)
国家重点研发计划项目(2016YFD0100204-15)
北京市农林科学院科技创新能力建设专项(KJCX20200113)。
