摘要
目的:探讨极低体重早产儿氧暴露后血清外泌体miR-30a、miR-34a变化趋势以及其与支气管肺发育不良(BPD)的关系。方法:选取2018年1月至2020年1月在衡水市妇幼保健院新生儿重症监护室住院>28 d的极低体重早产儿,包括56例BPD患儿(BPD组)和50例非BPD早产儿(非BPD组)。采集产后1、7、14、21、28 d新生儿循环血,分离血清外泌体,并采用实时荧光定量PCR法检测外泌体miR-30a、miR-34a水平。结果:产后14、21、28 d时BPD组外泌体miR-30a水平低于非BPD组,同时外泌体miR-34a水平高于非BPD组(P<0.05)。产后21、28 d时中重度BPD亚组外泌体miR-30a水平低于轻度BPD组,并且男性早产儿降低更明显(P<0.05);同时中重度BPD亚组外泌体miR-34a水平高于轻度BPD亚组(P<0.05),但是男性和女性相比差异无统计学意义(P>0.05)。绘制ROC曲线,产后28 d血清外泌体miR-30a、miR-34a诊断BPD的曲线下面积分别为0.868(95%CI:0.795~0.915)和0.798(95%CI:0.703~0.854)。结论:随着氧暴露时间的延长,极低体重BPD早产儿血清外泌体miR-30a水平逐渐降低,尤其是男性BPD患儿降低更明显,同时外泌体miR-34a水平逐渐升高。检测血清外泌体miR-30a、miR-34a表达有助于BPD诊断以及对病情进展的评估。
Objective:To investigate the relationship between the changes of serum exosomal miR-30a,miR-34a and bronchopulmonary dysplasia(BPD)in very low birth weight(VLBW)preterm infants during oxygen exposure.Methods:From January 2018 to January 2020,the preterm infants with VLBW hospitalized in Neonatal Intensive Care Unit of Hengshui Maternal and Child Health Care Hospital for more than 28 days were selected,including 56 cases of BPD(BPD group)and 50 cases of non BPD(non BPD group)preterm infants.Neonatal circulating blood at 1,7,14,21 and 28 days after delivery were collected.Serum exosomes were isolated,and the levels of exosomal miR-30a and miR-34a were detected by real-time quantitative PCR.Results:At the 14th,21th and 28th day after delivery,the levels of exosomal miR-30a of BPD group were lower than those of non BPD group,and the levels of exosomal miR-34a of BPD group were higher than those of non BPD group(P<0.05).At the 21th and 28th day after delivery,the level of exosomal miR-30a in the moderate&severe BPD subgroup was lower than that in the mild BPD group,while the male preterm infants decreased more significantly(P<0.05);meanwhile,the level of exosomal miR-34a in the moderate&severe BPD subgroup was higher than that of the mild BPD subgroup(P<0.05),but there was no significant difference between male and female(P>0.05).ROC curve showed that the area under the curve of exosomal miR-30a and miR-34a at the 28th day after delivery for the diagnosis of BPD were 0.868(95%CI:0.795-0.915)and 0.798(95%CI:0.703-0.854)respectively.Conclusion:With the prolongation of oxygen exposure time,the level of serum exosomal miR-30a in VLBW BPD preterm infants decreased gradually,especially in male BPD children,while the level of exosomal miR-34a increased gradually.Serum exosomal miR-30a and miR-34a would be helpful to the diagnosis of BPD and the evaluation of disease progression.
作者
牟佳
孙巨勇
牟娜
徐燕
MU Jia;SUN Juyong;MU Na;XU Yan(Department of Laboratory Medicine, Hengshui Maternal and Child Health Care Hospital, Hengshui 053000, China;Department of Laboratory Medicine, People's Hospital of Hengshui City, Hengshui 053000, China)
出处
《东南大学学报（医学版）》
CAS
2021年第3期298-305,共8页
Journal of Southeast University(Medical Science Edition)
