摘要
为探究目前海南地区罗非鱼无乳链球菌病的流行性,了解该地区无乳链球菌耐药情况,本研究2020年8月从海南地区部分渔业养殖场的病鱼中共分离获得15株无乳链球菌,对各分离菌株的血清型、主要毒力基因及耐药性进行检测,并对多重耐药情况进行分析。血清型检测结果显示,15株分离菌株的血清型与对照菌株A909一致,均为Ⅰa型。毒力基因检测结果显示,15株分离菌株均未检出毒力基因scpB,但该毒力基因在参考菌株人源无乳链球菌2603V/R中检出,而主要毒力基因cylE、sodA、gapC的检出率均为100%。药敏试验结果表明,15株分离菌株对甲氧胺嘧啶、磺胺异噁唑、复方新诺明、链霉素、新霉素、庆大霉素的耐药率达85%以上;对头孢克洛、头孢曲松、头孢哌酮、头孢拉定、卡那霉素、红霉素、利福平、克林霉素敏感性达80%以上;未发现对甲氧胺嘧啶、氟罗沙星、链霉素、新霉素、庆大霉素敏感的菌株。多重耐药检测结果表明,15株分离菌株均至少对4种药物表现耐药,其中6重及以上耐药的菌株占总分离菌株数的86.67%,并有2株为13重耐药菌株。本研究为海南地区鱼源无乳链球菌病的用药提供参考,为华南地区罗非鱼无乳链球菌的流行病学和疾病防控提供调查依据。
In order to explore the current prevalence and drug resistance of Streptococcus agalactiae isolated from tilapia of Hainan,in this study,15 strains of Streptococcus agalactiae were isolated from diseased fish in some tilapia farms in Hainan in August 2020.Serotypes,main virulence genes and multiple drug resistance of all isolates were detected.The serotype detection results showed that the serotype of 15 isolated was typeⅠa,consistent with the reference human strains A909.The results of virulence gene detection showed that scpB gene was only detected in human reference strain Streptococcus agalactiae 2603V/R except all isolates from tilapia.However,the detection rate of major virulence genes,such as cylE,sodA and gapC,was 100%.The drug sensitive test result indicated that the drug resistance rate of 15 isolates to methoxypyrimidine,sulfamisoxazole,cotrimoxazole,streptomycin,neomycin and gentamicin were more than 85%.The sensitive rate of cefcllor,ceftriaxone,cefoperazone,cefradine,kanamycin,erythromycin,rifampicin and clindamycin were more than 80%.No strains were found to be sensitive to methoxymidine,fleroxacin,streptomycin,neomycin and gentamicin.The results of multidrug resistance test showed that 15 isolated strains were resistant to 4 drugs at least,among all the isolates,86.67%were resistant to more than 6 kinds of antibiotics,and two of them were resistant to 13 kinds of antibiotics.This results provided reference for the application of antimicrobial agents for the treatment of tilapia infection of Streptococcus agalactiae in in Hainan,and also provided new data for the epidemiology and disease control of Streptococcus agalactiae of tilapia in South China.
作者
陈怀君
郭长明
朱善元
吴植
袁圣
王永娟
袁橙
潘勇
王晓晔
CHEN Huaijun;GUO Changming;ZHU Shanyuan;WU Zhi;YUAN Sheng;WANG Yongjuan;YUAN Cheng;PAN Yong;WANG Xiaoye(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Jiangsu Key Laboratory for High-Tech Research and Development of VeterinaryBiopharmaceuticals,Jiangsu Agri-Animal Husbandry Vocational College,Taizhou 225300,China;Hainan Evergreen Feed Industry Co.,Ltd.,Haikou 571152,China)
出处
《中国畜牧兽医》
CAS
北大核心
2021年第8期3058-3068,共11页
China Animal Husbandry & Veterinary Medicine
基金
江苏省高等学校自然科学研究面上项目(19KJB230005)
泰州市科技支撑计划(农业)项目(TN201916)
泰州市311高层次人才培养工程([2019]21号)
江苏农牧科技职业学院科研项目(NSF20190202)
江苏高校“青蓝工程”项目(苏教师函[2021]11号)。
关键词
鱼源无乳链球菌
血清型
毒力基因
多重耐药
Streptococcus agalactiae of tilapia
serotype
virulence gene
multiple drug resistance
