摘要
通过自主设计的同轴核-壳结构双层喷头及微流控系统,成功制备出一系列核-壳结构型多孔性生物活性硅酸钙基陶瓷微球,并通过对其核心层铜掺杂量的调控和外壳层微孔结构的剪裁,实现了该类微球核心层抗菌活性离子的可控缓释。采用X射线衍射分析明确了烧结后的陶瓷微球样品的物相组成和晶相结构。通过扫描电子显微镜观察发现使用该系统制备出的微球烧结后仍具有良好的球形形态及明确的核-壳结构,并且特定组分内部微孔结构符合设计要求。一系列模拟体内环境的微球浸泡实验系统研究了该类材料的体外离子释放行为和生物降解速率,研究结果显示该类陶瓷微球经特定层造孔后有快速的抗菌离子释放,并且释放速率可调可控。最后通过与金葡菌的体外共培养抑菌实验及细菌黏附实验等对微球的抗菌性能进行了研究,结果显示Cu10@Mg6-15μm组具有最佳的抑菌效果;优化壳层的多孔结构比单纯提高核层铜离子掺杂率更有利于活性离子释放。此次构建的壳层微孔优化型核-壳结构陶瓷微球,能够帮助解决抗感染离子可控缓释问题并带来长期抑菌的效果,为慢性骨髓炎等临床问题提供了潜在的治疗新材料。
In this experiment,a series of core-shell structured porous calcium silicate-based bioactive ceramic microspheres were successfully prepared through a self-designed coaxial double-layer nozzle and microfluidic system.By adjusting the doping amount of copper into the core layer and tailoring the structure of the shell layer,the release rate of antibacterial active ions of this material can be adjusted and controlled.X-ray diffraction(XRD)analysis was used to evaluate the phase composition of the sintered ceramic microspheres.Scanning electron microscope(SEM)analysis indicated that the microspheres prepared through this process remain a good spherical shape and a clear core-shell structure after sintering,and that the internal microstructure of specific components was constructed as designed.A series of immersion experiments systematically investigates the in vitro ion release behavior and biodegradation rate of these microspheres.Finally,the antibacterial properties of the microspheres were studied through in vitro co-culture with Staphylococcus aureus and bacterial adhesion experiments.The results showed that the Cu10@Mg6-15μm group obtained the best antibacterial effect.Thus,the optimization of the porous structure of the shell layer is more conducive to the release of active ions than simply increasing the copper ion doping rate of the core layer.This shell micropore optimized core-shell-structured bioceramic microspheres studied in this article can help solve the problem of controlled and sustained release of anti-infective ions and bring long-term antibacterial effects,providing potential treatment for clinical problems such as chronic osteomyelitis new material.
作者
叶硕
吴方汇
柯秀荣
王晓清
刘梦涛
杨贤燕
张雷
苟中入
杨国敬
YE Shuo;WU Fanghui;KE Xiurong;WANG Xiaoqing;LIU Mengtao;YANG Xianyan;ZHANG Lei;GOU Zhongru;YANG Guojing(Rui’an People’s Hospital&the 3rd Hospital Affiliated to Wenzhou Medical university,Rui’an 325200,China;Zhejiang-California International Nanosystems Institute,Zhejiang University,Hangzhou 310058,China)
出处
《材料科学与工程学报》
CAS
CSCD
北大核心
2021年第4期559-567,共9页
Journal of Materials Science and Engineering
基金
国家自然科学基金资助项目(81871775)
浙江省基础公益技术研究计划资助项目(LGF21H060002,LBY21H060001)
浙江省卫生健康委员会医药卫生科技资助项目(2020KY929)
温州市科技局基础性医疗卫生科技资助项目(Y20180307,Y20180862)。
关键词
离子选择性掺杂
多孔性陶瓷微球
核-壳结构
长效抗菌性
感染性骨缺损
Ion selective doping
Porous ceramic microspheres
Core-shell structure
Long-term antibacterial properties
Infective bone defect
