摘要
目的探讨常见过敏原致过敏反应易感性与过敏性皮肤病患者外周血HLA DRB1*0901(rs2395185)基因多态性的关系。方法选择广州地区皮肤科就诊患者304例,采用免疫印迹法检测患者血清19种过敏原,PCR测序检测外周血HLA DRB1*0901位点基因型,分析HLA DRB1*0901基因多态性与过敏原致过敏反应发病风险的关系。结果户尘螨过敏原检测结果阳性、阴性患者间rs2395185的G/G基因型、G/T基因型分布差异有统计学意义(P<0.05),G/G、G/T基因型与户尘螨过敏的发病风险有关(OR=2.940,95%CI:1.167~7.407;OR=2.889,95%CI:1.136~7.348)。轻度过敏与中重度过敏患者的G/T基因型分布差异有统计学意义(P<0.05),G/T基因型是过敏原反应严重程度的危险因素(OR=8.681,95%CI:1.077~69.989)。结论广州地区居民户尘螨皮肤过敏发病风险可能与其外周血HLA DRB1*09:01(rs2395185)位点G/G、G/T多态性有关,19种过敏原过敏的严重程度可能与rs2395185位点G/T多态性有关。
Objective To investigate the relationship between susceptibility to allergic reaction induced by common allergens and HLA DRB 1*0901(RS2395185)gene polymorphism in peripheral blood of patients with allergic skin disease.Methods A total of 304 patients who accepted treatment in department of dermatology in Guangzhou were selected.Nineteen kinds of allergens were detected by Western blotting.HLA DRB1*0901 genotypes were detected by PCR sequencing.The relationship between HLA DRB1*0901 gene polymorphism and the risk of allergic reaction induced by allergens was analyzed.Results The G/G and G/T genotypes of rs2395185 were significantly different between the positive and negative patients(P<0.05),and might be associated with the risk of house dust mite allergy(OR=2.940,95%CI:1.167~7.407;OR=2.889,95%C:1.136~7.348).There was significant difference in the distribution of G/T genotype between mild and moderate to severe allergic patients(P<0.05).G/T genotype may be a risk factor for the severity of allergic reaction(OR=8.681,95%CI:1.077-69.989).Conclusion The risk of skin allergy caused by Dermatophagoides pteronyssinus in population of Guangzhou may be related to the G/G and G/T polymorphisms of HLA DRB1*09:01(rs2395185),and the severity of allergy to 19 allergens may be related to the G/T polymorphism of rs2395185.
作者
周道平
区宇洁
薛晓芳
郭菁
甄英鹏
陆伟桃
张玲
莫桂玲
ZHOU Daoping;OU Yujie;XUE Xiaofang;GUO Jing;ZHEN Yingpeng;LU Weitao;ZHANG Ling;MO Guiling(Guangzhou Development District Hospital,Guangzhou 510730,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2021年第15期1972-1975,1981,共5页
The Journal of Practical Medicine
基金
广东省医学科研基金非资助项目(编号:B2018289)。
