摘要
龙牙百合Lilium brownii var.viridulum是药食同源百合,为江西省主产百合。以采自江西省宜春市万载县龙牙百合生产基地的腐烂组培苗为研究对象,因发病时茎叶甚至整株组培苗腐烂,故定义该病为"软腐病"。本研究对其病原菌进行分离纯化、菌落生长状态、菌丝、分生孢子、产孢结构等形态学观察、致病性检测和多基因位点序列鉴定。根据形态学观察初步鉴定病原菌为Acremonium sclerotigenum。进一步对病原菌的r DNA-ITS、LSU、SSU和β-tubulin多基因位点进行序列分析,发现其与A.sclerotigenum同源性最高并聚为一支。综上所述,确定引起龙牙百合软腐病的病原菌为产核枝顶孢A.sclerotigenum。
Lilium brownii var.viridulum is medicinal and edible lily mainly cultivated in Jiangxi Province.The tissue culture seedlings in some production regions were found to be attacked with a disease named as"soft rot".The leaves and stems or even the whole plants were rotten gradually.The pathogen was isolated and identified base on morphological characteristics,pathogenicity test,multilocus sequence alignment and phylogenic analysis.Morphological identification tentatively recognized the pathogen as Acremonium sclerotigenum.Further analysis of nucleotide sequences of rDNA internal transcribed spacer(ITS),large ribosomal subunits(LSU),small ribosomal subunits(SSU)and beta-tubulin(Tub2)of the fungal isolate indicated that it was highly clustered to A.sclerotignum clade in the phylogenetic tree.The pathogenic fungi was confirmed as A.sclerotignum.
作者
曾慧兰
刘佳蓉
李丽娜
黄琴
李润根
ZENG Hui-Lan;LIU Jia-Rong;LI Li-Na;HUANG Qin;LI Run-Gen(Department of Horticulture,College of Life Science and Environmental Resource,Yichun University,Yichun,Jiangxi 336000,China;Jiangxi Key Laboratory of Crop Growth and Regulation,Yichun University,Yichun,Jiangxi 336000,China;Department of Land Resources and Environment,College of Life Science and Environmental Resource,Yichun University,Yichun,Jiangxi 336000,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第7期1639-1647,共9页
Mycosystema
基金
江西省科技厅重点研发项目(20151BBF60050)
江西省作物生长发育调控实验室开放课题项目(KFJJ201704)
宜春学院博士科研启动项目(2103360117013)
2020年国家大学生创新创业训练计划项目(S202010417029)。
关键词
龙牙百合
软腐病
病原菌鉴定
产核枝顶孢
Lilium brownii var.viridulum
soft rot disease
pathogen identification
Acremonium sclerotigenum
