摘要
本研究利用愈创木酚和苯胺蓝固体培养基对菌株进行初筛,利用形态学和分子生物学对筛选出的菌株进行鉴定,以黄孢原毛平革菌Phanerochaete chrysosporium CGMCC 5.0776为对照,利用其对玉米秸秆进行预处理并测定木质素和纤维素的降解率,测定筛选菌株在预处理玉米秸秆过程中漆酶、锰过氧化物酶(manganese peroxidase,MnP)和木质素过氧化物酶(lignin peroxidase,LiP)活性。结果表明:利用愈创木酚和苯胺蓝固体培养基,从16株白腐真菌菌株中筛选出2株具有较高漆酶或MnP活性的菌株,鉴定其为桦栓孔菌Trametes betulina (L.) Pilát(ZT-153)和亚黑管孔菌Bjerkandera fumosa (Pers.) P.Karst.(ZT-307),测定T.betulinaZT-153和B.fumosaZT-307对玉米秸秆酸不溶木质素降解效率分别为13.60%和21.87%,较对照P. chrysosporium CGMCC 5.0776高1.58%和9.85%,对纤维素的降解率较低,分别为4.10%和4.50%。2株菌株在预处理玉米秸秆过程中,T. betulina ZT-153表现出漆酶和MnP活性,B.fumosa ZT-307只表现出LiP活性。其中B. fumosa ZT-307对玉米秸秆酸不溶木质素的降解效率最高,在秸秆资源的综合利用方面具有较好的潜力和应用前景。
Efficient lignin-degrading fungal strains were screened by using guaiacol-and aniline blue-containing solid media. Using Phanerochaete chrysosporium CGMCC 5.0776 as a control, cornstalk was pretreated with the selected strains. The degradation rates of lignin and cellulose, and the activities of laccase, manganese peroxidase(MnP) and lignin peroxidase(LiP) were determined. Two strains with higher activities of laccase or MnP were screened from 16 white-rot fungal strains. Based on morphology and molecular biology, they were identified as Trametes betulina(ZT-153) and B. fumosa(ZT-307). The lignin degradation efficiency on cornstalk was 13.60% by T. betulina ZT-153 and 21.87% by B. fumosa ZT-307, or respectively 1.58% and 9.85% as against P. chrysosporium CGMCC 5.0776, and the degradation rate of cellulose was 4.10% and 4.50% respectively. During the pretreatment of cornstalk, T. betulina ZT-153 showed active in degrading laccase and MnP, while B. fumosa ZT-307 only showed active in degrading LiP. Bjerkandera fumosa ZT-307 showed the highest lignin degradation ability, and it has potential and application prospects in comprehensive utilization of cornstalk resources.
作者
张芳芳
张桐
戴丹
张振豪
张波
李玉
ZHANG Fang-Fang;ZHANG Tong;DAI Dan;ZHANG Zhen-Hao;ZHANG Bo;LI Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第7期1869-1880,共12页
Mycosystema
基金
吉林省科技发展计划(20190201026JC)
科技部重点研发专项(2018YFE0107800)。
