摘要
An analytical method using gas chromatography isotope ratio mass spectrometry(GC-IRMS)combined with solid phase micro-extraction(SPME)was developed to measure the 613C values of six typical volatiles commonly occurring in wine(isoamyl acetate,2-octanone,limonene,2-phenylethanol,ethyl octanoate and ethyl decanoate)for the first time.SPME selected with a divinylbenzene/carboxen/polydimethylsiloxane fiber was combined with the GC-IRMS for pretreatment optimization.The optimized SPME parameters of extraction time,extraction temperature and salt concentration were 40 min,40℃ and 10%,respectively.The 613C values measured by SPME-GC-IRMS were in good agreement with those measured via elemental analyzer(EA)-IRMS and GC-IRMS.The differences range from 0.02‰to 0.44‰ with EA-IRMS and from 0 to 0.28‰ with GC-IRMS,indicating the high accuracy of the method.This newly established method measured the precision within 0.30‰ and was successfully validated to discriminate imported real wine samples with identical label but amazing price differences from different importers.
作者
Xinyu Jin
Limin Zhang
Shimin Wu
Mingquan Huang
Wenjuan Yu
Shanshan Zhang
金新宇;张立敏;吴时敏;黄明泉;喻文娟;张珊珊(Department of Food Science and Technology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai,China;Beijing Laboratory for Food Quality and Safety,Beijing Technology and Business University,Beijing,China;Instrumental Analysis Center,Shanghai JiaoTong University,Shanghai,China)
基金
supported by the fund of the Beijing Laboratory for Food Quality and Safety,Beijing Technology and Business University,China(No.FQS-201810)
Science and Technology Commission of Shanghai Municipality,China(No.19DZ2284200).
