摘要
为了解秦皇地区狐源沙门氏菌的致病性及耐药性,对23株秦皇地区的狐源沙门氏菌进行了研究。采用小鼠致病性试验、PCR检测毒力基因和KB纸片检测药物敏感性。结果显示,3株沙门氏菌均可致小鼠死亡,死亡率为20%~100%;23株沙门氏菌中有21株检测到毒力基因,分别检出2个~8个毒力基因,毒力基因sseC和sseL未检出,其余17种基因检出率为4.35%~65.22%,spvA、hisJ和stn基因检出率较高;23株沙门氏菌对6种~15种抗菌药物耐药,对14种抗菌药物耐药菌株数最多,占比26.09%(6/23),23株沙门氏菌对土霉素、替米考星和复方新诺明耐药率高达100%,对阿米卡星敏感率最高为30.43%(7/23)。结果表明,23株狐源沙门氏菌均具有致病性,并均携带多个毒力基因,耐药情况较为严重。
To study the pathogenicity and drug resistance of Salmonella from foxes in Qinhuangdao area,23 strains of Salmonella from foxes in the Qinhuangdao area were studied.The virulence gene was detected by PCR and the drug sensitivity was detected by KB paper.The results showed that all three strains of Salmonella can cause the death of mice,with a mortality rate of 20%-100%.21 strains of 23 strains of Salmonella detected virulence genes,with 2-8 virulence genes detected respectively,and the virulence genes sseC and sseL were not detected,the remaining 17 genes were 4.35%-65.22%,and the detection rates of spvA,hisJ and stn genes were high;23 strains of Salmonella were resistant to 6-15 kinds of antimicrobials,and resistance rate to 14 kinds of antimicrobials was the highest,accounting for 26.09%(6/23).The resistance rate of 23 Salmonella strains to terramycin,timicosin,and compound neoforman was 100%,and the sensitivity rate to amikacin was 30.43%(7/23).The results showed that all 23 strains of Salmonella were pathogenic and carried multiple virulence genes.
作者
刘勃兴
赵安奇
张雪佳
王利丽
吴同垒
冯岭
史秋梅
张志强
LIU Bo-xing;ZHAO An-qi;ZHANG Xue-jia;WANG Li-li;WU Tong-lei;FENG Ling;SHI Qiu-mei;ZHANG Zhi-qiang(Hebei Normal University of Science&Technology,Hebei Key Laboratory of Preventive Veterinary Medicine,Qinhuangdao,Hebei,066004,China;Laoting Agricultural and Rural Bureau,Tangshan,Hebei,063000,China)
出处
《动物医学进展》
北大核心
2021年第7期120-124,共5页
Progress In Veterinary Medicine
基金
秦皇岛市科技创新平台建设运行项目(201702B001)
河北省科技厅项目(18246629G)
河北省教育厅项目(ZD2017234)
农业部特种动物疫病重点试验室开放课题(01830)。
关键词
沙门氏菌
致病性
耐药性
狐
Salmonella
pathogenicity
drug resistance
fox
