摘要
为探究西藏地区葡萄表皮及根际土壤真菌群落结构组成,采用高通量测序技术对西藏芒康和林芝地区葡萄表皮及根际土壤样品进行真菌群落多样性分析。结果表明,芒康葡萄根际土壤(MS)真菌多样性最高,林芝葡萄(LG)真菌多样性最低。在门分类水平上,2个地区样品中的优势类群均为子囊菌门(Ascomycota),其相对丰度为76.06%~99.60%;在属分类水平上,不同样品中的优势菌群不同,林芝葡萄(LG)和芒康葡萄(MG)表皮样品中的优势菌属分别为枝孢属(Cladosporium)和汉逊酵母属(Hanseniaspora),其相对丰度分别为64.43%和31.59%,而林芝(LS)和芒康(MS)葡萄根际土壤样品中的优势菌属分别为镰胞菌属(Fusarium)和被孢霉属(Mortierella),其相对丰度分别为50.02%和20.16%;主成分分析表明,不同样品中真菌群落结构组成差异较大,不同地区葡萄表皮和根际土壤真菌群落结构组成相似;冗余分析表明,土壤中总钾(TK)、pH、总氮(TN)、总磷(TP)和速效氮(AN)对真菌群结构组成影响较大,而土壤电导率(EC)和有机质(SOM)影响不明显。综上所述,葡萄表皮和根际土壤富含丰富的真菌微生物,且不同地区样品间群落结构组成相似。西藏葡萄表皮及根际土壤微生物多样性的研究为开发本地特色微生物提供了理论依据。
To reveal the difference and diversity of fungal community structure on the surface of grape and rhizosphere soil from the different areas in Tibet,high-throughput sequencing technology was used to analyze the fungal diversity and community structure in grape and rhizosphere soil samples collected from Mangkang and Linzhi in Tibet.The results showed that the rhizosphere soil in Mangkang had a highest diversity of fungi,while the lowest on Linzhi grape surface.Ascomycota was the dominant phylum at phyla level,accounting for 76.06%~99.60%.At genus level,Cladosporium and Hanseniaspora were the dominant genus in LG and MG,respectively,accounting for 64.43%and 31.59%;while the dominant genus of LS and MS were Fusarium and Mortierella,respectively,accounting for 50.02%and 20.16%.Principal component analysis showed that the fungal community structure was significantly different in different samples,and the fungal community structure of grape epidermis and rhizosphere soil was similar in the different area.Redundancy analysis(RDA)showed that TK,pH,TN,TP and AN had a great influence on fungal community,but the effect of EC and SOM was not obvious.In conclusion,the surface of grape and rhizosphere soil microbial diversity from the different areas in Tibet were very rich,the fungal community structure was significantly similar in two areas.The study of grape and rhizosphere microbial diversity in Tibet could provide a theoretical basis for the utilization and development of characteristic microorganisms in this area.
作者
张二豪
赵润东
尹秀
蔡皓
禄亚洲
罗章
ZHANG Erhao;ZHAO Rundong;YIN Xiu;CAI Hao;LU Yazhou;LUO Zhang(Food Science College,Tibet Agriculture and Animal Husbandry University,Linzhi 860000,China)
出处
《食品工业科技》
CAS
北大核心
2021年第16期106-111,共6页
Science and Technology of Food Industry
基金
中央财政支持地方高校改革发展资金-生物技术特色专业建设项目(2019-007)。
关键词
葡萄表皮
根际土壤
高通量测序
相对丰度
多样性
grape surface
rhizosphere soil
high-throughput sequencing
relative abundance
diversity
