摘要
本文以楠木(Phoebe zhennan)为材料,利用抗乙酰赖氨酸富集和高灵敏质谱联用技术,对干旱胁迫处理的楠木叶片乙酰化蛋白质组进行分析鉴定,获得差异修饰蛋白1890个,差异修饰位点2759个。生物信息学分析表明,干旱条件下大多数乙酰化蛋白参与代谢过程。乙酰化主要发生在有催化活性和结合活性的相关蛋白上,且38.9%的乙酰化蛋白定位在叶绿体中。乙酰化蛋白参与碳代谢、糖酵解/糖异生、三羧酸循环以及谷胱甘肽代谢等生物学过程,尤其在谷胱甘肽S-转移酶以及氧化还原酶等相关的结构域显著富集。通过测定抗氧化酶活性发现干旱胁迫使超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性增加,而抗坏血酸过氧化物酶(APX)活性降低,推测抗坏血酸-谷胱甘肽循环以及谷胱甘肽代谢相关氧化还原酶等可能通过乙酰化响应干旱胁迫,调节抗氧化酶活性,消除活性氧对楠木的伤害。本研究报道了楠木响应干旱胁迫的差异乙酰化蛋白质组,为探索赖氨酸乙酰化在楠木干旱响应的作用机制提供参考。
In this study,nanmu(Phoebe zhennan)was used as material,label-free quantitative proteome approach was utilized to analyze and identify the acetylated proteome of nanmu leaves treated with drought stress,and finally a total of 1890 differentially modified proteins and 2759 differentially modified sites were obtained.Through intensive bioinformatic analyses,results show that most acetylated proteins were involved in metabolic processes under drought conditions,revealing the functional impacts of lysine acetylation in nanmu.Besides,acetylation mainly occurred in related proteins with catalytic and binding activities,and 38.9%of the differentially acetylated proteins were localized in chloroplasts.Moreover,acetylated proteins would participate in these biological processes,including carbon metabolism,glycolysis/gluconeogenesis,tricarboxylic acid cycle and glutathione metabolism,and they were particularly enriched in the related domains of glutathione S-transferase and oxidoreductase.Surprisingly,through the determination of anti-oxidase activity,it was found that the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)increased while ascorbate peroxidase(APX)decreased.Accordingly,it was inferred that glutathione-ascorbate(ASA-GSH)cycle and GSH metabolism related oxidoreductase had probably responded to drought stress through acetylation,in order to regulate antioxidant enzyme activities,and further prevent reactive oxygen species from harming nanmu.Finally,as for acetylome in nanmu,these data are available in this study,which would definitely provide a reference for later development in exploring the mechanism of lysine acetylation in response to drought in nanmu.
作者
赵丹
谢娜
赵德刚
ZHAO Dan;XIE Na;ZHAO Degang(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Institute of Agro-bioengineering,College of Life Sciences,Guizhou University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences,Guizhou Plant Conservation Technology Center,Guiyang 550006,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2021年第6期1371-1380,1381,共11页
Plant Physiology Journal
基金
贵州省科技计划项目(黔科合支撑[2020]1Y063号)
贵州省人才基地项目(RC2018-16)
贵州省高层次创新型人才培养项目(黔科合人才[2016]4003号)
中央引导地方科技发展专项资金(黔科中引地[2018]4001)
贵州大学引进人才科研项目(贵大人基合字[2018]05号)。
关键词
楠木
乙酰化蛋白质组
干旱胁迫
功能富集
差异表达蛋白
Phoebe zhennan
acetylate proteome
drought stress
function enrichment
differentially expressed protein
