摘要
以高原鳅(Triplophysa)属鱼类8个种群共75个个体的线粒体Cytb基因为标记,通过生物信息学分析高原鳅属种群遗传多样性和系统发育关系。结果表明,全长1140 bp的Cytb基因序列中共检测到527个多态位点,界定了61个单倍型,A+T含量(55.9%)高于G+C含量(44.2%)。8个种群单倍型多样度(Hd)范围在0.667~1.000之间,核苷酸多样度(Pi)在0.001~0.089之间,其中似鲶高原鳅(T.siluroides)遗传多样性水平最高(Hd=1.000,Pi=0.089),壮体高原鳅(T.robusta)遗传多样性水平最低(Hd=0.667,Pi=0.001);叶尔羌高原鳅(T.yarkandensis)与黄河高原鳅(T.pappenheimi)间的遗传距离最远(D=0.243),达里湖高原鳅(T.dalaica)与壮体高原鳅间的遗传距离最近(D=0.086);遗传分化系数表明:8个高原鳅种间存在着高度遗传分化(F_(st)>0.25),并且种群之间基因流较弱(N_(m)<1);分子系统发育进化树显示,细尾高原鳅与西藏高原鳅聚为一支,黄河高原鳅、似鲶高原鳅、壮体高原鳅、达里湖高原鳅四大种群聚为一支,贝氏高原鳅与叶尔羌高原鳅聚为一支。本研究为进一步对高原鳅属鱼类系统分类研究提供资料,以及对高原鳅保护措施提供了遗传学依据。
Mitochondrial Cytb gene of 75 individuals from 8 populations of the genus Triplophysa was used as marker to analyze the genetic diversity and phylogenetic relationship of the genus Triplophysa through bioinformatics.The results showed that the complete sequence of Cytb gene was 1140 bp,527 polymorphic sites were detected and 61 haplotypes were defined,the A+T content(55.9%)was higher than that of G+C(44.2%).Haplotype diversity(Hd)changed from 0.667 to 1.000,and nucleotide diversity(Pi)changed from 0.001 to 0.089.T.siluroides had the highest genetic diversity level(Hd=1.000,Pi=0.089),andT.robusta had the lowest genetic diversity level(Hd=0.667,Pi=0.001).The genetic distance ofT.yarkandensis and T.pappenheimi was the longest(D=0.243),while the genetic distance of T.dalaica and T.robusta was the closest(D=0.086).The genetic differenti ation index(F_(st)>0.25)and gene flow(N_(m)<1)indicated that there was a significant genetic differentiation among eight populations.The molecular phylogenetic evolution tree showed that one cluster comprised T.stenura and T.tibetana,the other cluster comprised T.pappenheimi,T.siluroides,T.robusta,and T.dalaica formed a branch.The third branch was T.bleekeri and T.yarkandensis.This study provides a reference for further studying the phylogenetic and genetic basis of Triplophysa,and a genetic basis for scientific and effective protection measures.
作者
赵桂研
权金强
李兰兰
罗志成
刘哲
Zhao Guiyan;Quan Jinqiang;Li Lanlan;Luo Zhicheng;Liu Zhe(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,730070)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第2期591-598,共8页
Genomics and Applied Biology
基金
甘肃省现代农业产业技术体系特种养殖产业体系项目(2019)资助。
