期刊文献+

CRISPR在食源性致病菌进化分析、检测分型及毒力耐药调控中的应用进展 被引量:3

Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens
原文传递
导出
摘要 规律成簇间隔的短回文重复序列(Clustered regularly interspaced short palindromic repeats,CRISPR)与其相关蛋白基因系统可通过限制基因的水平转移而有效防御噬菌体等外源基因元件的入侵,不同细菌之间的CRISPR结构有所差异。基于CRISPR系统的差异性,文中对近几年CRISPR在食源性致病菌进化分析、检测与分型、毒力与耐药中的应用研究进行概述,并对基于CRISPR序列特点开发的细菌检测分型方法以及CRISPR与食源性致病菌的毒力、耐药性之间的相关性进行重点总结分析。此外,文中探讨了CRISPR在进化分析、检测与分型、毒力与耐药应用中的不足,提出将CRISPR分型方法标准化、完善与扩充致病菌CRISPR数据库、进一步探究噬菌体与细菌之间的共进化关系等建议,为进一步探索CRISPR功能提供参考。 Clustered regularly interspaced short palindromic repeats(CRISPR) and its associated protein gene system can limit the horizontal gene transfer, thereby effectively preventing the invasion of foreign gene elements such as bacteriophages. CRISPR arrays of different bacteria are diverse. Based on the differences in the CRISPR system, this review summarizes the application of CRISPR in food-borne pathogen evolution analysis, detection and typing, virulence and antibiotic resistance in recent years. We also address bacterial detection typing method developed based on the characteristics of CRISPR arrays and the association of CRISPR with virulence and drug resistance of food-borne pathogens. The shortcomings of CRISPR in evolution, detection and typing, virulence and resistance applications are analyzed. In addition, we suggest standardizing CRISPR typing methods, improving and expanding the CRISPR database of pathogenic bacteria, and further exploring the co-evolution relationship between phages and bacteria, to provide references for further exploration of CRISPR functions.
作者 白芷烨 汪雯 吉小凤 肖英平 张世钦 王梓晨 李红梅 董庆利 Zhiye Bai;Wen Wang;Xiaofeng Ji;Yingping Xiao;Shiqin Zhang;Ziehen Wang;Hongmei Li;Qingli Dong(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,MOA Laboratory of Quality&Safety Risk Assessment for Agro-products(Hangzhou),Institute of Quality and Standard of Agricultural Products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China)
出处 《生物工程学报》 CAS CSCD 北大核心 2021年第7期2414-2424,共11页 Chinese Journal of Biotechnology
基金 国家重点研发计划(No.2019YFE0103800) 浙江省农业科学院省部共建农产品质量安全国家重点实验室开放基金项目(No.2010DS700124-KF2001)资助。
关键词 CRISPR 食源性致病菌 进化分析 检测与分型 毒力与耐药 CRISPR food-borne pathogens evolutionary analysis detection and typing virulence and antibiotic resistance
  • 相关文献

参考文献9

二级参考文献93

  • 1黄占景.插入序列IS_2的研究 Ⅰ.IS_2的插入方向与极性效应[J].Acta Genetica Sinica,1994,21(2):161-168. 被引量:2
  • 2王军,郑增忍,王晶钰.动物源性食品中沙门氏菌的风险评估[J].中国动物检疫,2007,24(4):23-25. 被引量:102
  • 3Allerberger F, Wagner M. Listeriosis: a resurgentfoodbome infection [J]. Clin. Microbiol. Infect., 2010, 16: 16-23.
  • 4Deltcheva E, Chylinski K, Sharma C M, et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III [J]. Nature, 2011,471:602-607.
  • 5Vzquez-Boland J, Kuhn M, Berche, et al. Listeria pathogenesis and molecular virulence determinants [J]. Clin. Microbiol. Rev., 2001, 14:584-640.
  • 6Hain T, Ghai R, Billion A, et al. Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes [J]. BMC Genomics, 2012, 13: 144.
  • 7Orsi R H, den Bakker H C, Wiedmann M. Listeria monocytogenes lineages: genomics, evolution, ecology, and phenotypic characteristics [J]. Int. J. Med. Microbiol., 2011, 301:79-96.
  • 8Doumith M, Cazalet C, Simoes N, et al. New aspects regarding evolution and virulence of Listeria monocytogenes revealed by comparative genomics and DNA arrays [J]. Infect. Immun., 2004, 72:1072-1083.
  • 9den Bakker H C, Didelot X, Fortes E D, et al. Lineage specific recombination rates and microevolution in Listeria monocytogenes [J]. BMC Evol. Biol., 2008, 8: 277.
  • 10Orsi R H, Sun Q, Wiedmann M. Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeriamonocytogenes [J]. BMC Evol. Biol., 2008, 8:233.

共引文献37

同被引文献43

引证文献3

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部