摘要
目的探索定量非对称回波的最小二乘估算法迭代水脂分离序列(iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence,IDEAL-IQ)定量评估维生素D干预兔糖尿病模型椎体骨髓脂肪含量的改变。材料与方法30只成年雄性新西兰兔随机分为3组,其中空白对照组(n=10)、单纯糖尿病组(n=10)、维生素D干预糖尿病组(n=10),兔糖尿病造模成功一周后,干预组连续4周每周400μg维生素D灌胃;相同时间节点对照组和单纯糖尿病组用生理盐水10 mL灌胃。3组兔模型在第0、4、8、12、16周行腰椎行矢状位磁共振检查(FSE-T2WI、FSE-T1WI、IDEAL-IQ),在IDEAL-IQ序列中Fat Fraction图像上测量腰椎感兴趣区内脂肪含量比值(FF%);在16周后处死所有大白兔,取腰5~7椎体做HE染色,计数骨髓内脂肪细胞;对各时间点3组兔腰椎体骨髓脂肪比(FF%)以及第16周HE染色下腰椎骨髓脂肪细胞计数采用重复测量的方差分析。将16周所得FF值及骨髓脂肪细胞含量行Pearson相关性分析。结果第16周单纯糖尿病组与维生素D干预组HE染色骨髓脂肪细胞计数较第0周明显增高,同时两组IDEAL-IQ测得16周椎体骨髓脂肪含量比较空白对照组的差异具有统计学意义(F=4.971,P<0.05;F=3.055,P<0.01);但在维生素D干预前后两组间FF值差异无统计学意义(t=2.390,P=0.06)。Pearson相关性分析结果显示第16周实验兔腰椎骨髓脂肪含量值与HE染色后的脂肪数目呈正相关(r=0.828,P<0.05)。结论IDEAL-IQ技术评价维生素D干预四氧嘧啶诱导兔糖尿病模型骨髓脂肪含量变化是可行的;维生素D干预16周内,骨髓脂肪含量改变呈现下降趋势。
Objective:Using iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence(IDEAL-IQ)to evaluate the effect of vitamin D supplement on the change of bone marrow fat content in vertebral body in alloxan-induced diabetic rabbits.Materials and Methods:Thirty New Zealand male rabbits were divided into 20 experienced rabbits(including alloxan-induced diabetic group(n=10),vitamin D intervention group(n=10),and 10 control rabbits,randomly.After the alloxan-induced diabetic model was modeling successfully,the intervention model was given vitamin D(400μg/week)orally for 4 weeks,and the control group and the simple diabetic group were given 10ml normal saline by gavage every week.After modeling,all rabbits were experienced the exam of magnetic resonance imaging(T1WI,T2WI,IDEAL-IQ)at 0th,4th,8th,12th,16th week,and all rabbits'vertebral fat content was calculate at GE AW 4.6 standard workstation.After 16 weeks,all rabbits were killed by air embolization,and the lumbar 5-7 vertebral body was taken for the adipocyte count with HE staining.The ratio of lumbar bone marrow to fat in control group,simple diabetes group and intervention group at each time point and the content of lumbar bone marrow adipocytes under HE staining were measured by repeated measurement ANOVA.Pearson correlation analysis was performed on FF value and bone marrow adipocyte content obtained at 16 weeks,and P<0.05 was considered statistically significant.Results:The percentage of bone marrow fat in the vertebral body measured by IDEA-IQ at 0th and 16th week was significantly different between the diabetic group and the vitamin D intervention groups(F=4.971,P<0.05;F=3.055,P<0.01),the control group has no statistical differences in vertebral fat fraction at every examination point;However,there was no statistically significant difference in FF value between the diabetic group and the vitamin D intervention group at 16th week(t=2.390,P=0.06).Pearson correlation analysis showed that there was a positive correlation between the fat fraction(FF)of lumbar bone marrow and the number of fat after HE staining at the 16th week in diabetic rabbits(r=0.828,P<0.05).Conclusions:IDEAL-IQ technique is feasible to evaluate the change of bone marrow fat content in alloxan-induced diabetic rabbits with vitamin D supplement.After 16 weeks of vitamin D supplement,the change of bone marrow fat content showed a decreasing trend.
作者
闫玉辰
查云飞
YAN Yuchen;ZHA Yunfei(Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《磁共振成像》
CAS
CSCD
北大核心
2021年第8期55-59,共5页
Chinese Journal of Magnetic Resonance Imaging
基金
国家自然科学基金(编号:81871332)。
关键词
糖尿病
骨髓
维生素D
脂肪定量
磁共振成像
diabetes mellitus
vertebral marrow
vitamin D
fat fraction
magnetic resonance imaging
