摘要
采用特异性引物PCR扩增方法对183份黄淮海麦区的小麦品种(系)的粒重基因TaCwi-A1、TaGw8-B1和TaGS-D1的等位变异进行分子鉴定,并结合2016—2017和2017—2018年度的千粒重表型数据,分析不同等位变异类型对小麦粒重的影响,从而找出优势基因型组合。结果表明,不同年份间参试品种(系)的千粒重差异达到极显著水平(P<0.01);TaCwi-A1位点上发现TaCwi-A1a和TaCwi-A1b两种等位变异,其分布频率分别为66.7%和33.3%;TaGw8-B1位点上TaGw8-B1a等位变异分布频率较高,为94.5%,而TaGw8-B1b等位变异分布频率极低,仅为5.5%;TaGS-D1位点上发现TaGS-D1a和TaGS-D1b两种等位变异,其分布频率分别为79.8%和20.2%。不同等位变异组合的品种千粒重存在显著差异(P<0.05),其中具有3个高千粒重等位变异组合TaCwi-A1a/TaGS-D1a/TaGw8-B1a品种的平均千粒重最高,与具有TaCwi-A1b/TaGS-D1a/TaGw8-B1a品种的平均千粒重差异不显著,但是显著高于其他组合(P<0.05)。TaCwi-A1a/TaGS-D1a/TaGw8-B1b基因型组合小麦品种(系)的平均千粒重最低。TaCwi-A1、TaGw8-B1和TaGS-D1位点上的不同等位变异均会导致小麦千粒重的显著变化,其中TaGw8-B1和TaGS-D1位点上的等位变异对小麦粒重的影响更为重要。在参试材料中没有发现具有3个低千粒重等位变异组合TaCwi-A1b/TaGS-D1b/TaGw8-B1b的品种,在7种不同等位变异组合中,具有3个高千粒重等位变异组合TaCwi-A1a/TaGS-D1a/TaGw8-B1a品种的平均千粒重最高,是优势基因型组合。
Grain weight is one of the most important yield traits in wheat.To accelerate the application process of dominant allelic variation of grain weight gene in wheat breeding,the allelic variation of grain weight gene was identified by using functional markers and the combinations of dominant grain weight genotypes were investigated the allelic variations of grain weight genes TaCwi-A1,TaGw8-B1,and TaGS-D1 in 183 wheat varieties(lines)from the Yellow-Huai-River Valley were identified by PCR amplification with specific primers.To identify the dominant genotype combinations,the effects of different allelic variation genotypes on wheat grain weight were studied by combining the phenotypic data of the 1000-grain weight(TGW)from 2016-2017 and 2017-2018.The results showed that the difference of TGW between different years was highly significant at P<0.01.Two alleles,TaCwi-A1a and TaCwi-A1b,were detected at TaCwi-A1 locus,with the frequencies of 66.7%and 33.3%,res-pectively.The frequency of TaGw8-B1a allele on TaGw8-B1 locus was up to 94.5%,while the frequency of TaGw8-B1b allele was only 5.5%.In addition,two alleles,TaGS-D1a and TaGS-D1b,were found in TaGS-D1 locus,and their frequencies were 79.8%and 20.2%,respectively.Further results indicated that there were significant differences in TGW among different allelic variation combinations at P<0.05.Among them,the average TGW of varieties with TaCwi-A1a/TaGS-D1a/TaGw8-B1a genotype was the highest.There was not significant difference in TGW between TaCwi-A1a/TaGS-D1a/TaGw8-B1a and TaCwi-A1b/TaGS-D1a/TaGw8-B1a genotype,but it was significantly higher than other genotypes at P<0.05.The average TGW of TaCwi-A1a/TaGS-D1a/TaGw8-B1b genotype was the lowest.The allelic variations at the loci of TaCwi-A1,TaGw8-B1,and TaGS-D1 all led to significant changes in TGW,and the allelic variations of TaGw8-B1 and TaGS-D1 loci were more important to TGW in wheat.There were no varieties with three low TGW allelic variation combinations TaCwi-A1b/TaGS-D1b/TaGw8-B1b in the tested materials.Among the seven different allele combinations,the average TGW of three high TGW allelic variation combi-nations TaCwi-A1a/TaGS-D1a/TaGw8-B1a was the highest,which was the dominant genotype combination.
作者
张福彦
程仲杰
陈晓杰
王嘉欢
陈锋
范家霖
张建伟
杨保安
ZHANG Fu-Yan;CHENG Zhong-Jie;CHEN Xiao-Jie;WANG Jia-Huan;CHEN Feng;FAN Jia-Lin;ZHANG Jian-Wei;YANG Bao-An(Isotope Institute Co.,Ltd,Henan Academy of Sciences/Henan Key Laboratory of Nuclear Agricultural Sciences,Zhengzhou,450015,Henan,China;Agronomy College,Henan Agricultural University/National Key Laboratory of Wheat and Maize Crop Science in Henan Province,Zheng-zhou,450002,Henan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第11期2091-2098,共8页
Acta Agronomica Sinica
基金
河南省小麦产业技术体系建设专项(Z2010-01-04)
河南省重点研发与推广专项(科技攻关)(202102110028)
河南省科学院基本科研业务专项(190604015)
省部共建小麦玉米作物学国家重点实验室开放课题(30500884)资助。
关键词
小麦
粒重基因
功能标记
等位变异
千粒重
wheat
grain weight gene
functional marker
allelic variation
TGW
