摘要
目的:探讨蛇六谷石油醚提取物(SLG)抑制白血病K562细胞增殖、促进凋亡和诱导分化的作用。方法:SLG处理K562细胞,同时加入ERK信号通路阻滞剂PD98059。采用MTT检测细胞活力,采用流式细胞术检测细胞凋亡率及分化相关抗原表达阳性的细胞比例,采用Western blot检测ERK1/2和p-ERK1/2的蛋白表达水平。结果:SLG 50、100、200 mg/L能够降低K562细胞的增殖活力,其抑制作用呈剂量依赖性(r=0.9997)。SLG能够提高细胞凋亡率及分化相关抗原CD11b、CD14和CD42b表达阳性的细胞比例,同时能够上调p-ERK1/2的表达。PD98059可部分逆转SLG促凋亡和分化的作用。结论:SLG通过ERK信号通路抑制K562细胞增殖、促进凋亡和诱导分化。
Objective: To investigate the role of petroleum ether extract of Rhizoma Amorphophalli( SLG) in inhibiting proliferation and promoting apoptosis and differentiation of leukemia K562 cells. Methods: K562 cells were processed by SLG and PD98059 which was the ERK signaling pathway blocker. Then cell vitality was tested by MTT.Cell apoptosis rate and positive percentage of antigen expression related with differentiation were detected by flow cytometry. The protein expression levels of ERK1/2 and p-ERK1/2 were detected by Western blot. Results: The proliferation activity of K562 was reduced by 50,100,200 mg/L SLG in a concentration dependent manner( r =0. 9997). The apoptosis rate and positive expression rate of CD11 b,CD14 and CD42 b which were related with differentiation were raised by SLG,as well as the expression of p-ERK1/2,while PD98059 could reverse the promoting effect of SLG on apoptosis and differentiation partially. Conclusion: SLG can inhibit the proliferation and promote apoptosis and differentiation of K562 cells through ERK signaling pathway.
作者
余潇苓
赵燕娜
尹利明
高瑞兰
潘磊
杜文喜
YU Xiao-Ling;ZHAO Yan-Na;YIN Li-Ming;GAO Rui-Lan;PAN Lei;DU Wen-Xi(Institute of Hematology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang Province,China;Department of Oncology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang Province,China;Key Laboratory of Bone Arthromyodynia,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2021年第4期1028-1033,共6页
Journal of Experimental Hematology
基金
浙江省自然科学基金项目(LQ19H290002,LY20H290004)
国家自然科学基金(81774068,81873113)
高等学校访问学者教师专业发展项目(FX2019019)。
关键词
蛇六谷
白血病
ERK信号通路
凋亡
细胞分化
细胞增殖
Rhizoma Amorphophalli
leukemia
ERK signaling pathway
apoptosis
cell differentiation
cell proliferation
