摘要
利用蛋白质燃烧法建立适用于全营养液态乳中蛋白质含量测定的方法。以杜马斯定氮仪作为分析仪器,探索测定蛋白质含量的最佳条件为氧气因子0.8 mL·mg^(-1)、氧气流量200 mL·min^(-1)、取样量100 mg,结果重复性试验RSD为0.9%,回收率为99.3%~101.6%。燃烧法与凯氏定氮法的测定结果对比显示二者无显著差别,表明燃烧法适用于测定全营养液态乳的蛋白质指标。
A method for the determination of protein content in total nutrition liquid milk was established by using protein combustion method.The optimum conditions of protein content were explored by using Dumas nitrogen analyzer as follows:the oxygen factor is 0.8 mL·mg^(-1),the oxygen flow is 200 mL·min^(-1) and the sample weight is100 mg.The results showed that the RSD of repeatability test was 0.9%,and the recovery rate was 99.3%~101.6%.There was no significant difference between combustion method and Kjeldahl method.The results show that the combustion method is suitable for the determination of protein index of total nutrition liquid milk.
作者
梁北梅
温恺嘉
王小妹
李咏华
武林贺
许文东
LIANG Beimei;WEN Kaijia;WANG Xiaomei;LI Yonghua;WU Linhe;XU Wendong(Guangzhou Hanfang Pharmaceutical Co.,Ltd.,Guangzhou 510240,China;National Engineering Research Center for Modernization of Extraction and Separation Process of Traditional Chinese Medicine,Guangzhou 510240,China;Guangdong Key Laboratory of Pharmaceutical Lipids,Guangzhou 510240,China)
出处
《现代食品》
2021年第12期210-213,共4页
Modern Food
基金
广东省药用脂质重点实验室(2020B1212070024)
2020年重大科技创新产业项目“基于临床营养的脂质原辅料及特医食品研发及产业化”。
关键词
燃烧法
杜马斯定氮仪
蛋白质
液态乳
氮含量测定
combustion method
Dumas nitrogen analyzer
protein
liquid milk
nitrogen content determination
